Measuring Small Molecule Binding to Escherichia coli AcrB by Surface Plasmon Resonance
| Autor: | Henrietta Venter, Rumana Mowla, Steven W. Polyak |
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| Přispěvatelé: | Polyak, Steven W., Mowla, Rumana, Venter, Henrietta |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Chemistry efflux pumps Protein subunit 030106 microbiology small molecule medicine.disease_cause antibiotic discovery Small molecule Receptor–ligand kinetics 03 medical and health sciences 030104 developmental biology binding kinetics medicine Biophysics Inner membrane antimicrobial resistance AcrA-AcrB-TolC Efflux Surface plasmon resonance Small molecule binding Escherichia coli surface plasmon resonance efflux pump inhibitors |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071601624 |
| Popis: | Antimicrobial resistance (AMR) is rapidly becoming one of the great healthcare challenges. A common mechanism employed by pathogenic bacteria to avoid the action of certain antibiotics is to overexpress efflux pumps that can extrude these drugs from the cell rendering them ineffective. Small molecule inhibitors that target bacterial efflux pumps provide a route toward reversing AMR. Here, we describe the application of surface plasmon resonance (SPR) technology to characterize protein:small molecule interactions between the inner membrane protein AcrB subunit of the Escherichia coli AcrA-AcrB-TolC efflux pump and its substrates and novel inhibitors. The SPR assay provides quantitative data about the kinetics of binding that can help guide the development of new chemotherapies to combat AMR. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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