Process development for the production of prebiotic galacto-oligosaccharides from lactose using β-galactosidase fromLactobacillus sp
| Autor: | Romana Zehetner, Barbara Splechtna, Thu-Ha Nguyen, Dietmar Haltrich, Hans Peter Lettner, Werner Lorenz |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_treatment
Cell Culture Techniques Oligosaccharides Lactose Applied Microbiology and Biotechnology Reaction rate chemistry.chemical_compound Bioreactors Lactobacillus medicine Food science Trisaccharide Sugar chemistry.chemical_classification biology Allolactose Probiotics Prebiotic General Medicine beta-Galactosidase biology.organism_classification Enzyme chemistry Biochemistry Molecular Medicine |
| Zdroj: | Biotechnology Journal. 2:480-485 |
| ISSN: | 1860-7314 1860-6768 |
| DOI: | 10.1002/biot.200600230 |
| Popis: | Galacto-oligosaccharides (GOS) are formed from lactose in discontinuous mode of conversion using beta-galactosidase from Lactobacillus sp. (beta-gal). The discontinuous process was optimized for technical application with regard to GOS yield, enzyme preparation, reaction temperature and substrate source. It proved to be advantageous to directly apply the crude cell-free enzyme extract for the conversion, since similar GOS yields and composition were obtained as when using the pure enzyme preparation, but expensive purification could be avoided. Reaction temperature was lowered to 17 degrees C to limit microbial contamination when using technical substrates. Thereby GOS yield decreased from 30% to 28% of total sugars and enzyme demand increased 2.7-fold. Whey permeate was compared to buffered lactose solution as a substrate source. The initial reaction rate was found to be 1.8 times higher for the whey permeate substrate; however, GOS yield was slightly lower (approximately 25% of total sugar at 17 degrees C) mainly due to smaller amounts of allolactose[beta-D-Galp-(1--6)-D-Glc] and the trisaccharide beta-D-Galp-(1--6)-D-Lac formed. |
| Databáze: | OpenAIRE |
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