Effects of long-term exposure of human periodontal ligament cells to milk and other solutions
| Autor: | Nijole A. Remeikis, Shiang-Chih Huang, Jon C. Daniel |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Materials science
business.industry Cell Survival Periodontal Ligament medicine.medical_treatment Dentistry Balanced salt solution Fibroblasts Contact lens Contact lens solutions Milk medicine Periodontal fiber Animals Humans Contact Lens Solutions Tissue Preservation Isotonic Solutions business General Dentistry Saline Cells Cultured |
| Zdroj: | Journal of endodontics. 22(1) |
| ISSN: | 0099-2399 |
| Popis: | Periodontal ligament (PDL) cells cultured from healthy extracted human teeth were exposed to milk, Alcon Opti-Free contact lens solution, K-Mart contact lens solution, saline, and Hank's balanced salt solution. The appearance and rate of loss of the cells from the culture dishes were recorded over time at both room temperature (20 degrees C) and 4 degrees C. The results indicated that saline was superior to either of the contact lens solutions in its ability to maintain the vitality of the PDL cells. Milk at 4 degrees DC provided good short-term viability , but cells did not remain attached after 48 h. At 20 degrees C, however, milk resulted in a 24.4% retention of cells after 72 h. Hank's balanced salt solution was the best storage media, with 46.8% of sells remaining attached after 72 h of exposure. This study supports milk as a good short-term storage medium for maintaining the vitality of PDL cells in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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