Altered expression of long non-coding RNA and mRNA in mouse cortex after traumatic brain injury
| Autor: | Hongrong Zhang, Xiaochuan Sun, Li Jiang, Jianjun Zhong, Zhijian Huang, Yong Jiang, Chongjie Cheng, Jianhua Peng, Junchi He, Han Liu, Fang Cao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Traumatic brain injury Poison control Down-Regulation Gene Expression 03 medical and health sciences Mice Cortex (anatomy) Brain Injuries Traumatic medicine Animals RNA Messenger Molecular Biology Cerebral Cortex Messenger RNA cDNA library business.industry Sequence Analysis RNA General Neuroscience Gene Expression Profiling RNA Computational Biology medicine.disease Molecular biology Fold change Long non-coding RNA Up-Regulation Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure RNA Long Noncoding Neurology (clinical) business Developmental Biology |
| Zdroj: | Brain research. 1646 |
| ISSN: | 1872-6240 |
| Popis: | The present study aims to detect the altered lncRNA expression in the mouse cortex after traumatic brain injury (TBI). We also simultaneously detected the altered mRNA profile to further analyze the possible function of lncRNA.C57BL/6 mice (n=18) were used to construct a controlled cortical impact model. At 24h post-TBI, the cortex around injury site was collected and the total RNA was extracted to construct the cDNA library. RNA sequencing (RNA-seq) was carried out followed by RT-PCR for confirmation. Bioinformatic analysis (including GO analysis, KEGG pathway and co-expression analysis) also were performed.A total of 64,530 transcripts were detected in the current sequencing study, in which 27,457 transcripts were identified as mRNA and 37,073 transcripts as lncRNA. A total of 1580 mRNAs (1430 up-regulated and 150 down-regulated) and 823 lncRNAs (667 up-regulated and 156 down-regulated) were significantly changed according to the criteria ( (|)log2((fold change))|1 and P0.05). These altered mRNAs were mainly related to inflammatory and immunological activity, metabolism, neuronal and vascular network. The expression of single lncRNA may be related with several mRNAs, and so was the mRNA. Also, a total of 360 new mRNAs and 8041 new lncRNAs were identified. The good reproducibility and reliability of RNA-seq were confirmed by RT-PCR.Numerous lncRNAs and mRNAs were significantly altered in mouse cortex around the injury site 24h after TBI. Our present data may provide a promising approach for further study about TBI. |
| Databáze: | OpenAIRE |
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