Hexane Extract of Orthosiphon stamineus Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells
| Autor: | Yong-Woon Kim, Jong-Yeon Kim, Yoon Jung Choi, Hae-Jung Lee, Kyu Chang Won, So-Young Park, Jong-Keun Son |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Orthosiphon stamineus Type 2 diabetes medicine.disease_cause Glucose-stimulated insulin secretion Insulin mRNA chemistry.chemical_compound Internal medicine Diabetes mellitus Islet Studies and Transplantation Medicine Phosphatidylinositol Protein kinase B Glucotoxicity PI3K/AKT/mTOR pathway biology business.industry Insulin biology.organism_classification medicine.disease Endocrinology chemistry Original Article business Oxidative stress |
| Zdroj: | Diabetes & Metabolism Journal |
| ISSN: | 2233-6079 |
| Popis: | Background: Hyperglycemia, a characteristic feature of diabetes, induces glucotoxicity in pancreatic β-cells, resulting in further impairment of insulin secretion and worsening glycemic control. Thus, preservation of insulin secretory capacity is essential for the management of type 2 diabetes. In this study, we evaluated the ability of an Orthosiphon stamineus (OS) extract to prevent glucotoxicity in insulin-producing cells. Methods: We measured insulin mRNA expression and glucose-stimulated insulin secretion (GSIS) in OS-treated INS-1 cells after exposure to a high glucose (HG; 30 mM) concentration. Results: The hexane extract of OS elevated mRNA expression of insulin as well as pancreatic and duodenal homeobox-1 of INS1 cells in a dose-dependent manner. The hexane OS extract also increased the levels of phosphorylated phosphatidylinositol 3-kinase (PI3K) in a concentration-dependent manner. Additionally, Akt phosphorylation was elevated by treatment with 100 and 200 μmol of the hexane OS extract. Three days of HG exposure suppressed insulin mRNA expression and GSIS; these ex pressions were restored by treatment with the hexane OS extract. HG elevated peroxide levels in the INS-1 cells. These levels were unaffected by OS treatment under both normal and hyperglycemic conditions. Conclusion: Our results suggested that the hexane extract of OS elevates insulin mRNA expression and prevents glucotoxicity induced by a 3-day treatment with HG. This was associated with the activation of PI-3K and Akt. |
| Databáze: | OpenAIRE |
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