Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution
| Autor: | Yokimiko David, Hee Taek Kim, Jae Jun Song, Sung Min Hyun, Jong Hyun Choi, Si Jae Park, Jeong Chan Joo, Tae Uk Khang, Kyoung Hee Kang, Kei Anne Baritugo, Ju Hyun Yu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gamma-aminobutyrate Decarboxylation Co-utilization Mutant lcsh:QR1-502 Bioengineering Xylose Empty fruit bunch medicine.disease_cause Applied Microbiology and Biotechnology lcsh:Microbiology Corynebacterium glutamicum law.invention 03 medical and health sciences chemistry.chemical_compound law medicine Gamma aminobutyrate Escherichia coli gamma-Aminobutyric Acid Strain (chemistry) Research 030104 developmental biology Biochemistry chemistry Fruit Fermentation Recombinant DNA Biotechnology |
| Zdroj: | Microbial Cell Factories Microbial Cell Factories, Vol 17, Iss 1, Pp 1-12 (2018) |
| ISSN: | 1475-2859 |
| Popis: | Background Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gamma-aminobutyrate (GABA), a versatile bulk chemical used in food and pharmaceutical industry, is also used as a key monomer for nylon 4. GABA can be biologically produced by decarboxylation of glutamate. Results In this study, we examined high glutamate-producing Corynebacterium glutamicum strains as hosts for enhanced production of GABA from glucose and xylose as carbon sources. An Escherichia coli gadB mutant with a broad pH range of activity and E. coli xylAB genes were expressed under the control of a synthetic H36 promoter. When empty fruit bunch (EFB) solution was used as carbon source (45 g/L glucose and 5 g/L xylose), 12.54 ± 0.07 g/L GABA was produced by recombinant C. glutamicum H36GD1852 expressing E. coli gadB mutant gene and xylAB genes. Batch fermentation of the same strain resulted in the production of 35.47 g/L of GABA when EFB solution was added to support 90 g/L glucose and 10 g/L xylose. Conclusions This is the first report of GABA production by recombinant C. glutamicum strains from co-utilization of glucose and xylose from EFB solution. Recombinant C. glutamicum strains developed in this study should be useful for an efficient and sustainable production of GABA from lignocellulosic biomasses. Electronic supplementary material The online version of this article (10.1186/s12934-018-0977-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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