Age, sex, and race influence single-strand break repair capacity in a human population
| Autor: | Ngozi Ejiogu, Andrzej R. Trzeciak, Larry J. Brant, Michele K. Evans, Janice Barnes, Alan B. Zonderman, Kamala Foster |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male DNA Repair DNA damage DNA repair Coefficient of variation Population DNA Single-Stranded Biology Biochemistry Peripheral blood mononuclear cell White People Article Andrology chemistry.chemical_compound Sex Factors stomatognathic system Physiology (medical) Humans education education.field_of_study Racial Groups Age Factors Middle Aged Comet assay White (mutation) Black or African American stomatognathic diseases chemistry Gamma Rays Immunology Leukocytes Mononuclear Female Comet Assay DNA DNA Damage |
| Zdroj: | Free radical biologymedicine. 45(12) |
| ISSN: | 0891-5849 |
| Popis: | Recently, we developed an improved comet assay protocol for evaluating single-strand break repair capacity (SSB-RC) in unstimulated cryopreserved human peripheral blood mononuclear cells (PBMCs). This methodology facilitates control of interexperimental variability [A.R. Trzeciak, J. Barnes, M.K. Evans, A modified alkaline comet assay for measuring DNA repair capacity in human populations. Radiat. Res. 169 (2008) 110-121]. The fast component of SSB repair (F-SSB-RC) was assessed using a novel parameter, the initial rate of DNA repair, and the widely used half-time of DNA repair. The slow component of SSB repair (S-SSB-RC) was estimated using the residual DNA damage after 60 min. We have examined repair of gamma-radiation-induced DNA damage in PBMCs from four age-matched groups of male and female whites and African-Americans between ages 30 and 64. There is an increase in F-SSB-RC with age in white females (P |
| Databáze: | OpenAIRE |
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