The human papillomavirus type 16 (HPV-16) E5 protein sensitizes human keratinocytes to apoptosis induced by osmotic stress
| Autor: | Kirsten Kabsch, Angel Alonso |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Keratinocytes
Cancer Research Programmed cell death Osmotic shock Recombinant Fusion Proteins Hypertonic Solutions Drug Resistance Apoptosis Cytochrome c Group Biology Transfection p38 Mitogen-Activated Protein Kinases Culture Media Serum-Free Mice Osmotic Pressure Genetics medicine Animals Humans Sorbitol Fluorescent Antibody Technique Indirect Molecular Biology Cells Cultured Cell Line Transformed integumentary system Cytochrome c Oncogene Proteins Viral Fibroblasts Virology Cell biology Mitochondria Enzyme Activation HaCaT medicine.anatomical_structure Cell culture Caspases biology.protein Mitogen-Activated Protein Kinases Poly(ADP-ribose) Polymerases Keratinocyte Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Oncogene. 21(6) |
| ISSN: | 0950-9232 |
| Popis: | We have studied the role of the HPV-16 E5 protein in apoptosis, using HaCaT cell lines stably transfected with either E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as control. When subjected to a hyperosmolar concentration of sorbitol, HaCaT/E5 cells respond with cytochrome c release, activation of caspase-3, -8, and -9, and PARP-cleavage, showing that the mitochondria and death-receptor mediated apoptotic pathways are involved in subsequent cell death. Similar effects are observed for the control cells only after extended sorbitol treatment. Thus, E5-expressing cells are more sensitive to osmotic stress, perhaps because of modifications of the cellular membranes caused by this strongly hydrophobic molecule. |
| Databáze: | OpenAIRE |
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