Effects of buffer composition, pH and temperature on oxygen binding by planorbid snail haemoglobins

Autor:	K. Naude, W.J. van Aardt
Rok vydání:	1990
Předmět:	Ecology chemistry.chemical_element Bohr effect Biology biology.organism_classification Oxygen chemistry.chemical_compound chemistry Sephadex Ionic strength Biomphalaria glabrata Animal Science and Zoology Composition (visual arts) PMSF Oxygen binding Nuclear chemistry
Zdroj:	African Zoology; Vol 25, No 1 (1990); 18-25
ISSN:	0254-1858 2224-073X 1562-7020
Popis:	The oxygen-binding properties of the haemoglobins of four species of freshwater planorbid snails Bulinus africanus, Biomphalaria glabrata, Bulinus tropicus and Heliosoma trivolvis were examined. Samples chromatographed on Sephadex G75 in CO-saturated buffer (0,1 mol dm−3 MgCl2, 0,05 mol dm−3 Tris-HCl, 0,5 mmol dm−3 phenylmethyl sulfonyl fluoride (PMSF), 1,0 mmol dm−3Dithioerytritol, ionic strength 0,3) gave the best stability against methaemoglobin formation. The highest haemoglobin oxygen affinity (P50 = 0,35 mmHg) was measured with MgCl2-buffer with a Hill coefficient, nmax, of 1,16. An nmax value of 2,2 was obtained with snail buffer (4 mmol dm−3 KCl, 50 mmol dm
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cee55d630c8732dfcd7a5c09a35790c5 https://doi.org/10.1080/02541858.1990.11448183 Zobrazit plný text záznamu