Rapid Detection of Gut Microbial Metabolite Trimethylamine N-Oxide for Chronic Kidney Disease Prevention
| Autor: | Chien-Cheng Wang, Chih-Hsuan Wang, Hung-Wei Yang, Yi-Hsuan Chu, Yu-Chun Chang, You-Lin Tain |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Colorectal cancer
Metabolite Clinical Biochemistry Trimethylamine Trimethylamine N-oxide chronic kidney disease (CKD) Pharmacology Gut flora Article Methylamines chemistry.chemical_compound nanoenzyme medicine Humans Renal Insufficiency Chronic Whole blood trimethylamine N-oxide (TMAO) biology gut microbiota Oxides General Medicine Atherosclerosis medicine.disease biology.organism_classification Gastrointestinal Microbiome Manganese Compounds chemistry colorimetry Quantitative analysis (chemistry) TP248.13-248.65 Biotechnology Kidney disease |
| Zdroj: | Biosensors Volume 11 Issue 9 Biosensors, Vol 11, Iss 339, p 339 (2021) |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios11090339 |
| Popis: | The gut microbiota plays a critical role in chronic kidney disease (CKD) and hypertension. Trimethylamine-N-oxide (TMAO) and trimethylamine (TMA) are gut microbiota-derived metabolites, and both are known uraemic toxins that are implicated in CKD, atherosclerosis, colorectal cancer and cardiovascular risk. Therefore, the detection and quantification of TMAO, which is a metabolite from gut microbes, are important for the diagnosis of diseases such as atherosclerosis, thrombosis and colorectal cancer. In this study, a new “colour-switch” method that is based on the combination of a plasma separation pad/absorption pad and polyallylamine hydrochloride-capped manganese dioxide (PAH@MnO2) nanozyme was developed for the direct quantitative detection of TMAO in whole blood without blood sample pretreatment. As a proof of concept, a limit of quantitation (LOQ) of less than 6.7 μM for TMAO was obtained with a wide linear quantification range from 15.6 to 500 μM through quantitative analysis, thereby suggesting potential clinical applications in blood TMAO monitoring for CKD patients. |
| Databáze: | OpenAIRE |
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