Gold nanocrystal-mediated sliding of doublet DNA origami filaments
| Autor: | Maximilian J. Urban, Chao Zhou, Thomas Weiss, Klas Lindfors, Anton Kuzyk, Steffen Both, Na Liu |
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| Přispěvatelé: | Max Planck Institute for Intelligent Systems, University of Stuttgart, Department of Neuroscience and Biomedical Engineering, University of Cologne, Aalto-yliopisto, Aalto University |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Surface Properties Science General Physics and Astronomy Kinesins Metal Nanoparticles FOS: Physical sciences 02 engineering and technology macromolecular substances 010402 general chemistry Antiparallel (biochemistry) 01 natural sciences Microtubules General Biochemistry Genetics and Molecular Biology Fluorescence spectroscopy Article chemistry.chemical_compound Microscopy Electron Transmission Microtubule Fluorescence Resonance Energy Transfer DNA origami Physics - Biological Physics lcsh:Science Nanoscopic scale Multidisciplinary General Chemistry DNA 021001 nanoscience & nanotechnology 0104 chemical sciences Cross-Linking Reagents Nanocrystal chemistry Biological Physics (physics.bio-ph) Biophysics Nanoparticles Nucleic Acid Conformation lcsh:Q Gold Elongation 0210 nano-technology Physics - Optics Optics (physics.optics) |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-7 (2018) Nature Communications |
| DOI: | 10.48550/arxiv.2105.00804 |
| Popis: | Sliding is one of the fundamental mechanical movements in machinery. In macroscopic systems, double-rack pinion machines employ gears to slide two linear tracks along opposite directions. In microscopic systems, kinesin-5 proteins crosslink and slide apart antiparallel microtubules, promoting spindle bipolarity and elongation during mitosis. Here we demonstrate an artificial nanoscopic analog, in which gold nanocrystals can mediate coordinated sliding of two antiparallel DNA origami filaments powered by DNA fuels. Stepwise and reversible sliding along opposite directions is in situ monitored and confirmed using fluorescence spectroscopy. A theoretical model including different energy transfer mechanisms is developed to understand the observed fluorescence dynamics. We further show that such sliding can also take place in the presence of multiple DNA sidelocks that are introduced to inhibit the relative movements. Our work enriches the toolbox of DNA-based nanomachinery, taking one step further toward the vision of molecular nanofactories. Kinesin, a motor protein, moves along filaments in a walk-like fashion to transport cargo to specific places in the cell. Here, the authors developed an analogous, artificial system consisting of nanoparticles moving along DNA filaments. |
| Databáze: | OpenAIRE |
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