Transcriptional regulation of the sulfate-starvation-induced gene sfnA by a σ 54-dependent activator of Pseudomonas putida
| Autor: | Hisakazu Yamane, Hideaki Nojiri, Atsushi Kouzuma, Takayuki Endoh, Toshio Omori, Hiroshi Habe |
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| Rok vydání: | 2007 |
| Předmět: |
Transcription
Genetic Operon Molecular Sequence Data Mutant lac operon Sulfides Microbiology Bacterial Proteins Gene cluster Transcriptional regulation Sulfhydryl Compounds Gene Binding Sites Base Sequence biology Pseudomonas putida Sulfates Activator (genetics) Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Biochemistry Trans-Activators RNA Polymerase Sigma 54 Gene Deletion Heat-Shock Response |
| Zdroj: | Microbiology. 153:3091-3098 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.2007/008151-0 |
| Popis: | The sigma(54)-dependent transcriptional regulator SfnR is essential for the use of dimethyl sulfone (DMSO(2)) as a sulfur source by Pseudomonas putida DS1. SfnR binds three SfnR-binding sites (sites 1, 2 and 3) within an intergenic region of the divergently transcribed sfnAB and sfnFG gene clusters. The site 1 region, proximal to the sfnF gene, is indispensable for the expression of the sfnFG operon, which encodes components of DMSO(2) monooxygenase. We investigated the transcriptional regulation of the sfnAB operon and possible functions of the sfnA gene. RT-PCR analysis revealed that the sfnAB gene cluster, which is similar to homologues of the acyl-CoA dehydrogenase family, was transcribed as an operon, and its expression was regulated by SfnR under conditions of sulfate starvation. Deletion analyses using lacZ as a reporter demonstrated that the region up to at least -138 bp from the transcription start point of sfnA (containing sites 2 and 3) was necessary for the expression of the sfnAB operon. A growth test of the sfnA-disrupted mutant revealed the possibility that sfnA may be involved in the use of methanethiol as a sulfur source. |
| Databáze: | OpenAIRE |
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