Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) terpolymer production from volatile fatty acids using engineered Ralstonia eutropha
| Autor: | Yung-Hun Yang, Hun-Suk Song, Jeong-Jun Yoon, Yun-Gon Kim, Tae-Rim Choi, Da-Hye Yi, Shashi Kant Bhatia, Soo-Yeon Yang, Hye-Rim Jung, Jong-Min Jeon, Christopher J. Brigham |
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| Rok vydání: | 2019 |
| Předmět: |
Polymers
Ralstonia 02 engineering and technology Biochemistry Polyhydroxyalkanoates Butyric acid 03 medical and health sciences chemistry.chemical_compound Structural Biology Copolymer Organic chemistry Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology General Medicine Polymer Nuclear magnetic resonance spectroscopy Fatty Acids Volatile 021001 nanoscience & nanotechnology biology.organism_classification Monomer chemistry Propionate Genetic Engineering 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 138:370-378 |
| ISSN: | 0141-8130 |
| Popis: | One of the advantages of microbial synthesis of polyhydroxyalkanoates (PHAs) is the production of diverse polymers with different properties by the addition of different monomers, such as 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx). Considering the number of possible variables, terpolymers can have more variations than copolymers. In this study, we aimed to synthesize the terpolymer P(3HB-co-3HV-co-3HHx) from volatile fatty acids such as propionate and butyrate using the recombinant Ralstonia eutropha strain (Re2133/pCB81), containing deletions in the phaB1, phaB2, and phaB3 genes, and overexpression of synthetic PHA operon (phaC2, phaA, phaJ). This strain produced terpolymers depending on the ratio of two different carbon sources, namely, propionic and butyric acids; however, wild type R. eutropha could not produce the same type of polymer. The incorporation of 3-hydroxyvalerate and 3-hydroxyhexanoate monomers was confirmed by gas chromatography and H-nuclear magnetic resonance spectroscopy, and the parameters affecting the terpolymer composition were obtained based on regression. In addition, the thermal analysis showed that this terpolymer has properties different from those of the copolymer, obtained from the same composition of volatile acids. Depending on the ratio of two volatile acids, the composition of the terpolymer can be regulated resulting in different properties. |
| Databáze: | OpenAIRE |
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