Studies on Early-Appearing Interferon in Vitro I. Production of Endotoxin-Induced Interferon by Mouse Spleen Cells Cultured in Vitro
| Autor: | Osamu Yasui, Masaaki Masuzumi, Sigeyasu Kobayashi |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Time Factors
Acid Phosphatase Newcastle disease virus Spleen General Biochemistry Genetics and Molecular Biology Cell Line Mice chemistry.chemical_compound L Cells In vivo Interferon Culture Techniques Escherichia coli medicine Animals Inducer Glucuronidase biology Acid phosphatase Hydrogen-Ion Concentration Molecular biology In vitro Culture Media Endotoxins Titer medicine.anatomical_structure chemistry Puromycin Dactinomycin biology.protein Interferons Lysosomes medicine.drug |
| Zdroj: | Experimental Biology and Medicine. 131:487-494 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-131-33908 |
| Popis: | SummaryMouse spleen cells cultured in vitro produced at least two kinds of interferon (IF) in response to inoculation with E. coli endotoxin. The early-appearing IF showed its peak titer about 2 hr after the inoculation of inducer, while the late-appearing IF possessed its maximum titer at 6–8 hr. Their properties were generally similar to those of endotoxin-induced IF in mouse plasma or spleen in vivo. The production of endotoxin-induced IF in mouse plasma or spleen was in parallel with the appearance of lysosomal enzymes, β-glucuronidase and acid phosphatase. In the case of cultured spleen cells in vitro, such parallelism was not observed at all. The production of early-IF in vitro was not affected by treatment with actinomycin D (10μg/ml), but inhibited almost completely by puromycin (50 μg/ml). Both inhibitors caused almost complete inhibition of the production of late-IF. |
| Databáze: | OpenAIRE |
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