Enzymatic enrichment of arachidonic acid from Mortierella single-cell oil

Autor: Yumi Minamigawa, Kenichi Higashiyama, Shigeaki Fujikawa, Yuji Shimada, Akio Sugihara, Kengo Akimoto, Yoshio Tominaga, Sadao Komemushi
Rok vydání: 1998
Předmět:
Zdroj: Journal of the American Oil Chemists' Society. 75:1213-1217
ISSN: 0003-021X
DOI: 10.1007/s11746-998-0314-2
Popis: An attempt was made to enrich arachidonic acid (AA) from Mortierella single-cell oil, which had an AA content of 25%. The first step involved the hydrolysis of the oil with Pseudomonas sp. lipase. A mixture of 2.5 g oil, 2.5 g water, and 4000 units (U) Pseudomonas lipase was incubated at 40°C for 40 h with stirring at 500 rpm. The hydrolysis was 90% complete after 40 h, and the resulting free fatty acids (FFA) were extracted with n-hexane (AA content, 25%; recovery of AA, 91%). The second step involved the selective esterification of the fatty acids with lauryl alcohol and Candida rugosa lipase. A mixture of 3.5 g fatty acids/lauryl alcohol (1:1, mol/mol), 1.5 g water, and 1000 U Candida lipase was incubated at 30°C for 16 h with stirring at 500 rpm. Under these conditions, 55% of the fatty acids were esterified, and the AA content in the FFA fraction was raised to 51% with a 92% yield. The long-chain saturated fatty acids in the FFA fraction were eliminated as urea adducts. This procedure raised the AA content to 63%. To further elevate the AA content, the fatty acids were esterified again in the same manner with Candida lipase. The repeated esterification raised the AA content to 75% with a recovery of 71% of its initial content.
Databáze: OpenAIRE
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