Altered proteome of a Burkholderia pseudomallei mutant defective in short-chain dehydrogenase affects cell adhesion, biofilm formation and heat stress tolerance
| Autor: | Manas Chongsa-nguan, Taksaon Duangurai, Nitaya Indrawattana, Tipparat Thiangtrongjit, Pornpan Pumirat, Amporn Rungruengkitkun, Onrapak Reamtong |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Melioidosis
Burkholderia pseudomallei Proteome Mutant lcsh:Medicine ATP-binding cassette transporter Biology Microbiology General Biochemistry Genetics and Molecular Biology Heat stress Short-chain dehydrogenase 03 medical and health sciences medicine Biofilm formation Molecular Biology 030304 developmental biology 0303 health sciences 030306 microbiology General Neuroscience lcsh:R Biofilm General Medicine biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification bacterial infections and mycoses Deletion Mutagenesis Hsp70 Adhesion bacteria General Agricultural and Biological Sciences |
| Zdroj: | PeerJ, Vol 8, p e8659 (2020) PeerJ |
| ISSN: | 2167-8359 |
| Popis: | Burkholderia pseudomallei is a Gram-negative bacillus that causes melioidosis and is recognized as an important public health problem in southeast Asia and northeast Australia. The treatment of B. pseudomallei infection is hampered by resistance to a wide range of antimicrobial agents and no vaccine is currently available. At present, the underlying mechanisms of B. pseudomallei pathogenesis are poorly understood. In our previous study, we reported that a B. pseudomallei short-chain dehydrogenase (SDO; BPSS2242) mutant constructed by deletion mutagenesis showed reduced B. pseudomallei invasion and initial intracellular survival. This indicated that SDO is associated with the pathogenesis of melioidosis. In the present study, the role of B. pseudomallei SDO was further investigated using the SDO deletion mutant by a proteomic approach. The protein profiles of the SDO mutant and wild-type K96243 were investigated through gel-based proteomic analysis. Quantitative intensity analysis of three individual cultures of the B. pseudomallei SDO mutant revealed significant down-regulation of five protein spots compared with the wild-type. Q-TOF MS/MS identified the protein spots as a glutamate/aspartate ABC transporter, prolyl-tRNA synthetase, Hsp70 family protein, quinone oxidoreductase and a putative carboxypeptidase. Functional assays were performed to investigate the role of these differentially expressed proteins in adhesion to host cells, biofilm induction and survival under heat stress conditions. The SDO deletion mutant showed a decreased ability to adhere to host cells. Moreover, biofilm formation and the survival rate of bacteria under heat stress conditions were also reduced in the mutant strain. Our findings provide insight into the role of SDO in the survival and pathogenesis of B. pseudomallei at the molecular level, which may be applied to the prevention and control of B. pseudomallei infection. |
| Databáze: | OpenAIRE |
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