Anti-biofilm and wound-healing activity of chitosan-immobilized Ficin
| Autor: | Victoria Koroleva, Andrey G. Porfiryev, Nafis Akhmetov, Valeriy Artyukhov, M. G. Holyavka, Mariya N. Agafonova, Milana N. Chirkova, Diana R. Baidamshina, Mikhail I. Bogachev, Elena Semenova, Valeriya V. Shubina, T. V. Baltina, Svetlana Pankova, O Sachenkov, Elena Y. Trizna, Olga S. Vasileva, Airat R. Kayumov |
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| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Immobilized enzyme 02 engineering and technology Microbial Sensitivity Tests Biochemistry Microbiology Chitosan 03 medical and health sciences chemistry.chemical_compound Structural Biology In vivo Molecular Biology 030304 developmental biology 0303 health sciences Drug Carriers Wound Healing biology Biofilm Substrate (chemistry) General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology biology.organism_classification Enzymes Immobilized In vitro Ficain Kinetics chemistry Solubility Biofilms Proteolysis 0210 nano-technology Wound healing Bacteria |
| Zdroj: | International journal of biological macromolecules. 164 |
| ISSN: | 1879-0003 |
| Popis: | Biofouling is among the key factors slowing down healing of acute and chronic wounds. Here we report both anti-biofilm and wound-healing properties of the chitosan-immobilized Ficin. The proposed chitosan-adsorption approach allowed preserving ~90% of the initial total activity of the enzyme (when using azocasein as a substrate) with stabilization factor of 4.9, and ~70% of its specific enzymatic activity. In vitro, the chitosan-immobilized Ficin degraded staphylococcal biofilms, this way increasing the efficacy of antimicrobials against biofilm-embedded bacteria. In vivo, in the presence of Ficin (either soluble or immobilized), the S.aureus-infected skin wound areas in rats reduced twofold after 4 instead of 6 days treatment. Moreover, topical application of the immobilized enzyme resulted in a 3-log reduction of S. aureus cell count on the wound surfaces in 6 days, compared to more than 10 days required to achieve the same effect in control. Additional advantages include smoother reepithelisation, and new tissue formation exhibiting collagen structure characteristics closely reminiscent of those observed in the native tissue. Taken together, our data suggest that both soluble and immobilized Ficin appear beneficial for the treatment of biofilm-associated infections, as well as speeding up wound healing and microbial decontamination. |
| Databáze: | OpenAIRE |
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