Overexpression of long non-coding RNA TUG1 predicts poor prognosis and promotes cancer cell proliferation and migration in high-grade muscle-invasive bladder cancer
| Autor: | Jaroslav Juracek, Ondrej Slaby, Michal Fedorko, Renata Kleinova, Dalibor Pacík, Marek Svoboda, Zuzana Ozanova, Robert Iliev, Michal Staník, Jan Dolezel |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Apoptosis Biology Real-Time Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Biomarkers Tumor Tumor Cells Cultured Gene silencing Humans Neoplasm Invasiveness RNA Messenger RNA Small Interfering Cell Proliferation Neoplasm Staging Muscle Neoplasms Bladder cancer Cell growth Reverse Transcriptase Polymerase Chain Reaction Cell Cycle General Medicine Cell cycle medicine.disease Prognosis Gene Expression Regulation Neoplastic 030104 developmental biology Real-time polymerase chain reaction Urinary Bladder Neoplasms Cell culture 030220 oncology & carcinogenesis Cancer cell Cancer research Disease Progression Biomarker (medicine) Female RNA Long Noncoding |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 37(10) |
| ISSN: | 1423-0380 |
| Popis: | Long non-coding RNA TUG1 is involved in the development and progression of a variety of tumors. Little is known about TUG1 function in high-grade muscle-invasive bladder cancer (MIBC). The aims of our study were to determine expression levels of long non-coding RNA TUG1 in tumor tissue, to evaluate its relationship with clinico-pathological features of high-grade MIBC, and to describe its function in MIBC cells in vitro. TUG1 expression levels were determined in paired tumor and adjacent non-tumor bladder tissues of 47 patients with high-grade MIBC using real-time PCR. Cell line T-24 and siRNA silencing were used to study the TUG1 function in vitro. We observed significantly increased levels of TUG1 in tumor tissue in comparison to adjacent non-tumor bladder tissue (P |
| Databáze: | OpenAIRE |
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