Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon
| Autor: | Ariane Nzouankeu, Muhamed-Kheir Taha, Eva Hong, Ala-Eddine Deghmane, Eric-Walter Pefura-Yone, Aude Terrade, Véronique Penlap Beng, Marie-Christine Fonkoua, Serges Tchatchouang, Richard Njouom, Mélanie Denizon, Suzie Moyo Tetang Ndiang |
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| Přispěvatelé: | Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), Centre National de Référence des Méningocoques et Haemophilus influenzae - National Reference Center Meningococci and Haemophilus influenzae (CNR), Institut Pasteur [Paris], Université de Yaoundé I, Centre Hospitalier Essos [Yaoundé, Cameroun], Jamot hospital, This work was supported by the United States Department of Health and Human Services [grant number 6 DESP060001-01-01], the Institut Pasteur and the Institut Pasteur International Network (RIIP) through Traineeship Grants Calmette and Yersin., Institut Pasteur [Paris] (IP) |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Serotype MESH: Drug Resistance Microbial Antibiotic resistance Antibiotics MESH: beta-Lactamases Quinolones Polymerase Chain Reaction 0302 clinical medicine MESH: Child Ampicillin Cameroon 030212 general & internal medicine Child Respiratory Tract Infections MESH: Microbial Sensitivity Tests Respiratory tract infections Drug Resistance Microbial General Medicine Anti-Bacterial Agents 3. Good health Ciprofloxacin Infectious Diseases Child Preschool Female MESH: Haemophilus Infections medicine.drug Microbiology (medical) MESH: Mutation Haemophilus Infections Adolescent Respiratory tract infection medicine.drug_class 030106 microbiology Microbial Sensitivity Tests Biology beta-Lactamases lcsh:Infectious and parasitic diseases Microbiology 03 medical and health sciences MESH: Anti-Bacterial Agents Haemophilus Haemophilus species medicine Humans lcsh:RC109-216 MESH: Adolescent MESH: Humans MESH: Quinolones MESH: Child Preschool typing MESH: Polymerase Chain Reaction MESH: Haemophilus influenzae MESH: Cameroon biology.organism_classification rpoB [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Haemophilus influenzae MESH: Male Typeing Whole genome sequencing MESH: Respiratory Tract Infections Mutation MESH: Ampicillin MESH: Female |
| Zdroj: | International Journal of Infectious Diseases, Vol 100, Iss, Pp 12-20 (2020) International Journal of Infectious Diseases International Journal of Infectious Diseases, Elsevier, 2020, 100, pp.12-20. ⟨10.1016/j.ijid.2020.08.040⟩ International Journal of Infectious Diseases, 2020, 100, pp.12-20. ⟨10.1016/j.ijid.2020.08.040⟩ |
| ISSN: | 1201-9712 1878-3511 |
| Popis: | Objectives: To identifyHaemophilus species and characterise the antimicrobial susceptibility of isolates from patients with respiratory tract infections (RTIs) in Cameroon. Methods: Isolates (n = 95) were from patients with RTIs obtained from two hospitals in Yaoundé, Cameroon. Isolates were identified by biochemical assay, a polymerase chain reaction (PCR)-based method, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF), and whole genome sequencing. Antibiotic minimum inhibitory concentrations were determined by E-test. Results: Haemophilus influenzae (H. influenzae) was the most prevalent species, varying from 76.8 to 84.2% according to the different methods. The isolates were mainly non-typeable (n = 70, 96%). Three H. influenzae isolates were capsulated (b, e and f). The isolates were genetically diverse and 40 unique sequence types were identified, including 11 new ones. Resistance to ampicillin was observed among 52 of 94 (55.3%), and 14 of the 52 (26.9%) produced TEM-1 β-lactamase. PBP3 mutations occurred in 40 of 52 (76.9%) ampicillin-resistant isolates. Eleven isolates were chloramphenicol-resistant, with eight of 10 (80%) producing chloramphenicol acetyltransferase. Four Haemophilus isolates were rifampicin-resistant, with two mutations in rpoB gene. Five isolates were ciprofloxacin-resistant and harboured mutations in the quinolone-resistance-determining regions of gyrA and parC genes. Conclusion: TheH. influenzae isolates were highly diverse and showed high levels of antibiotic resistance. H. influenzae serotype b is still circulating in the post-vaccination era. |
| Databáze: | OpenAIRE |
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