Honokiol Suppression of Human Epidermal Growth Factor Receptor 2 (HER2)-Positive Gastric Cancer Cell Biological Activity and Its Mechanism
| Autor: | Jianmin Xu, Guoxin Mao, Yidan Yan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Honokiol
Receptor ErbB-2 Cell Apoptosis 030204 cardiovascular system & hematology Lignans 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Stomach Neoplasms Tumor Cells Cultured medicine Humans RNA Messenger Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation Cell growth Biphenyl Compounds General Medicine Cell cycle Molecular biology medicine.anatomical_structure Matrix Metalloproteinase 9 chemistry Case-Control Studies 030220 oncology & carcinogenesis Cancer cell Phosphatidylinositol 3-Kinase |
| Zdroj: | Medical Science Monitor. 26 |
| ISSN: | 1643-3750 |
| DOI: | 10.12659/msm.923962 |
| Popis: | BACKGROUND The purpose of our study was to determine the effects and mechanisms of honokiol on human epidermal growth factor receptor 2 (HER2)-positive gastric cancer cells by in vitro study. MATERIAL AND METHODS We measured HER2 expression in different gastric cancer cell lines by real-time quantitative polymerase chain reaction (RT-qPCR) and western blot (WB) assay. Cell proliferation, apoptosis, and cell cycle were evaluated by cell-counting kit 8 and flow cytometry assays. The invading cell numbers and wound-healing rates were measured by transwell and wound-healing assays. Phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), P21, and matrix metalloproteinase (MMP)-9 proteins and messenger ribonucleic acid (mRNA) expression were measured by WB and RT-qPCR assay. HER2 protein expression was evaluated by cellular immunofluorescence. RESULTS Honokiol suppressed cell proliferation via increasing cell apoptosis, invasion, and migration with dose dependence. By WB and RT-qPCR assays, compared with the control group, PI3K, AKT, P21, and MMP-9 proteins and mRNA expression were significantly different (P |
| Databáze: | OpenAIRE |
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