| Popis: |
Table ST1. List of oligonucleotides and siRNAs used in this study. Table ST2. List of antibodies used in this study. Figure S1. Sequence alignment of OTUD6A and OTUD6B (isoform 1) performed using Clustal omega software. Figure S2. RNA silencing to OTUD2 and OTUD5 in NSCLC cells does not affect the protein synthesis rate. Figure S3. Comassie blue staining of a representative SDS PAGE gel loaded with 40 μg of proteins obtained from total cell lysate (1), nuclear fraction (2), postmitochondrial fraction (3), postribosomal fraction (4) and ribosomes (5). Note the typical enrichment in low molecular weigh bands (ribosomal proteins) of the ribosomal fraction. Figure S4. OTUD6B coimmunoprecipitates with eIF4G and other eIF4F components. Figure S5. Schematic (Top) and sequence alignment (Bottom) of OTUD6B isoforms. Large spiral: coiled coil domain. Figure S6. Evidence of allelic mutation (arrow) of the OTUD6B catalytic core in HCC827. Figure S7. OTUD6B-1 and OTUD6B-2 cause cell death when overexpressed in NSCLC cells. Figure S8. Hematoxylin/Eosin staining of 8μm-thick, frozen slides of representative normal lung. |
