Quantitation of catalpol in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its pharmacokinetic study

183.0 for catalpol and m/z 364.3-->167.0 for aucubin (IS) in the positive ion mode with electrospray ionization (ESI) source. Calibration curve was linear over the concentration range of 10-20,000 ng/mL. The mean recovery was 76.5+/-5.2% and the matrix effect ranged from -5.1 to 13.0%. The intra- and inter-day precisions were less than 6.3 and 14.6%, respectively, and the accuracy was within +/-5.6%. Catalpol was stable in possible conditions of storing and handling. The validated method has been successfully applied to determine the plasma concentration of catalpol for a pharmacokinetic study of catalpol after oral administration of 50 mg/kg to rats. -->
ISSN: 1570-0232
Přístupová URL adresa: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ced26ea700bd673f097f74ffce3aecf7
https://doi.org/10.1016/j.jchromb.2009.08.047
Rights: CLOSED
Přírůstkové číslo: edsair.doi.dedup.....ced26ea700bd673f097f74ffce3aecf7
Autor: Duanyun Si, Changxiao Liu, Yuan Gu, Rong Lu
Rok vydání: 2009
Předmět:
Zdroj: Journal of Chromatography B. 877:3589-3594
ISSN: 1570-0232
Popis: A sensitive, rapid and specific liquid chromatography/tandem mass spectrometry (LC/MS/MS) assay has been established for the quantitation of catalpol in rat plasma. Plasma samples were treated by precipitating protein with methanol and were chromatographed by a Diamonsil C(18) column (150 mm x 4.6 mm I.D., 5 microm) with the mobile phase consisting of methanol and 10 mM ammonium formate (30:70, v/v). The selected reaction monitoring (SRM) transitions were performed at m/z 380.1-->183.0 for catalpol and m/z 364.3-->167.0 for aucubin (IS) in the positive ion mode with electrospray ionization (ESI) source. Calibration curve was linear over the concentration range of 10-20,000 ng/mL. The mean recovery was 76.5+/-5.2% and the matrix effect ranged from -5.1 to 13.0%. The intra- and inter-day precisions were less than 6.3 and 14.6%, respectively, and the accuracy was within +/-5.6%. Catalpol was stable in possible conditions of storing and handling. The validated method has been successfully applied to determine the plasma concentration of catalpol for a pharmacokinetic study of catalpol after oral administration of 50 mg/kg to rats.
Databáze: OpenAIRE
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