Promoter trapping of a novel medium-chain Acyl-CoA oxidase, which is induced transcriptionally during Arabidopsis seed germination
| Autor: | Laurent Nussaume, Nichole Bechtold, Dawn Williams, Mark A. Hooks, Peter J. Eastmond, Catherine Sarrobert, Peter R. Lange, Ian A. Graham |
|---|---|
| Přispěvatelé: | Department of Biology, Centre for Novel Agricultural Products, University of York [York, UK], University of Wales, University of Glasgow, Unité de recherche Génétique et amélioration des plantes (GAP), Institut National de la Recherche Agronomique (INRA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Plant Environmental Physiology and Stress Signaling (PEPSS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
0106 biological sciences
Transcription Genetic Mutant Arabidopsis BIOTECHNOLOGIE OXYDASE ACYL-COA biosynthèse 01 natural sciences Biochemistry Substrate Specificity Hypocotyl Acyl-CoA oxidase biotechnologie végétale Cloning Molecular Promoter Regions Genetic ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification 0303 health sciences Oxidase test acide gras promoteur Peroxisome Recombinant Proteins [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Seeds plante escherichia coli Oxidoreductases récepteur DNA Complementary expression génique Molecular Sequence Data Germination Biology Isozyme 03 medical and health sciences Amino Acid Sequence mutant [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Molecular Biology 030304 developmental biology Base Sequence Arabidopsis Proteins arabidopsis thaliana Fatty acid Cell Biology biology.organism_classification Gene Expression Regulation chemistry Acyl-CoA Oxidase mutation 010606 plant biology & botany |
| Zdroj: | Journal of Biological Chemistry 44 (275), 34375-34381. (2000) Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2000, 275 (44), pp.34375-34381. ⟨10.1074/jbc.M004945200⟩ Journal of Biological Chemistry, 2000, 275 (44), pp.34375-34381. ⟨10.1074/jbc.M004945200⟩ |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M004945200⟩ |
| Popis: | The first step of peroxisomal fatty acid beta-oxidation is catalyzed by a family of acyl-CoA oxidase isozymes with distinct fatty acyl-CoA chain-length specificities. Here we identify a new acyl-CoA oxidase gene from Arabidopsis (AtACX3) following the isolation of a promoter-trapped mutant in which beta-glucuronidase expression was initially detected in the root meristem. In acx3 mutant seedlings medium-chain acyl-CoA oxidase activity was reduced by 95%, whereas long- and short-chain activities were unchanged. Despite this reduction in activity lipid catabolism and seedling development were not perturbed. AtACX3 was cloned and expressed in Escherichia coli. The recombinant enzyme displayed medium-chain acyl-CoA substrate specificity. Analysis of beta-glucuronidase activity in acx3 revealed that, in addition to constitutive expression in the root axis, AtACX3 is also up-regulated strongly in the hypocotyl and cotyledons of germinating seedlings. This suggests that beta-oxidation is regulated predominantly at the level of transcription in germinating oilseeds. After the discovery of AtACX3, the Arabidopsis acyl-CoA oxidase gene family now comprises four isozymes with substrate specificities that encompass the full range of acyl-CoA chain lengths that exist in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|