Lack of in Vivo Antibody Dependent Cellular Cytotoxicity with Antibody Containing Gold Nanoparticles
| Autor: | Mark E. Davis, Marya Ahmed, Dorothy W. Pan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Biomedical Engineering
Metal Nanoparticles Mice Nude Pharmaceutical Science Antineoplastic Agents Bioengineering chemical and pharmacologic phenomena Mice Immune system In vivo Cell Line Tumor medicine Animals Humans Panitumumab Pharmacology Antibody-dependent cell-mediated cytotoxicity Cetuximab biology Chemistry Communication Organic Chemistry Antibody-Dependent Cell Cytotoxicity Antibodies Monoclonal Xenograft Model Antitumor Assays Molecular biology 3. Good health Cytolysis Colloidal gold biology.protein Cancer research Gold Antibody Biotechnology medicine.drug |
| Zdroj: | Bioconjugate Chemistry |
| Popis: | Antibody-dependent cellular cytotoxicity (ADCC) is a cytolytic mechanism that can elicit in vivo antitumor effects and can play a significant role in the efficacy of antibody treatments for cancer. Here, we prepared cetuximab, panitumumab, and rituximab containing gold nanoparticles and investigated their ability to produce an ADCC effect in vivo. Cetuximab treatment of EGFR-expressing H1975 tumor xenografts showed significant tumor regression due to the ADCC activity of the antibody in vivo, while the control antibody, panitumumab, did not. However, all three antibody containing nanoparticles are not able to suppress tumor growth in the same in vivo mouse model. The antibody containing nanoparticles localized in the tumors and did not suppress the immune function of the animals, so the lack of tumor growth suppression of the cetuximab containing nanoparticle suggests that immobilizing antibodies onto a nanoparticle significantly decreases the ability of the antibody to promote an ADCC response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|