The comparison genomics analysis with glioblastoma multiforme (GBM) cells under 3D and 2D cell culture conditions
| Autor: | Qian Xue, Liang Ma, Qingchong Qiu, Jun Zou, Biao-Yang Lin, Changchun Zhou, Duo Zhang, Lei Gao, Li Binjie, Bin Zhang, Luo Yichen, Yuting Li, Huayong Yang, Mengfei Yu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Polyesters Cell Culture Techniques Biology 03 medical and health sciences 0302 clinical medicine Colloid and Surface Chemistry Downregulation and upregulation Gene expression Tumor Cells Cultured Humans Gene Regulatory Networks Physical and Theoretical Chemistry Gene Genomics Surfaces and Interfaces General Medicine In vitro Gene Expression Regulation Neoplastic 030104 developmental biology Tumor progression Cell culture 030220 oncology & carcinogenesis Cancer research Signal transduction DNA microarray Glioblastoma Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 172:665-673 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2018.09.034 |
| Popis: | GBM, the most common and aggressive malignant primary brain tumors which needs new research approach to reveal the underline molecular mechanism of tumor progression. The 3D in vitro tumor model can be a simple and effective way to study tumor characteristics with ability to replicate of the tumor milieu. In the current study, we adopted the DNA microarray to analyze the gene expression of GBM tumor cells cultured under 2D cell culture flasks and 3D PLA porous scaffolds for 4,7 and 14 days. For 14 day old cultures, 8117 and 3060 genes expression were upregulated and downregulated respectively. Further KEGG pathway analysis revealed, the upregulated genes were mainly enriched/involved in PPAR and PI3K-Akt signaling pathways whereas the downregulated genes were mainly contributed in metabolism, ECM related and TGF-beta pathways. Thus, our approach of establishing 3D in vitro tumor model provides realistic results and proves itself a powerful tool for understanding the inner nature of GBM and can be considered as potential platform for drug screening. |
| Databáze: | OpenAIRE |
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