Substrate specificity of proline-4-hydroxylase: Chemical and enzymatic synthesis of 2S,3R,4S-epoxyproline

Autor:	Victor Lee, Christopher J. Schofield, J. Kenneth Robinson, Jack E. Baldwin, Robert A. Field, Christopher C. Lawrence
Rok vydání:	1994
Předmět:	Bicyclic molecule biology Stereochemistry Chemistry Organic Chemistry Substrate (chemistry) Enzymatic synthesis biology.organism_classification Biochemistry Streptomyces Dioxygenase Drug Discovery Substrate specificity Proline
Zdroj:	Tetrahedron Letters. 35:4649-4652
ISSN:	0040-4039
DOI:	10.1016/s0040-4039(00)60753-0
Popis:	The substrate specificity of L -proline-4-hydroxylase, a 2-oxoglutarate dependent dioxygenase from Streptomyces griseoviridus P8648, was investigated. Preliminary assays measuring turnover of 2-oxoglutarate indicated 3,4-dehydro- L -proline was an efficient substrate. The identity of the product was shown to be 2 S ,3 R ,4 S -epoxy- L -proline by comparison with synthetic 2 S ,3 R ,4 S - and 2 S ,3 S ,4 R -epoxy- L -prolines.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cecc9da9c7447e6455f65b733513ebbd https://doi.org/10.1016/s0040-4039(00)60753-0 Zobrazit plný text záznamu