Genomic characterization of environmental Pseudomonas aeruginosa isolated from dental unit waterlines revealed the insertion sequence ISPa11 as a chaotropic element
| Autor: | Brian Boyle, Antony T. Vincent, Marie-Christine Groleau, Steve J. Charette, Luca Freschi, Irena Kukavica-Ibrulj, Fabrice Jean-Pierre, Julie Jeukens, Eric Déziel, Jean Barbeau, Annie Leduc, Roger C. Levesque, Jean-Guillaume Emond-Rheault |
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| Přispěvatelé: | Université Laval [Québec] (ULaval), Institut Universitaire de Cardiologie et de Pneumologie de Québec (IUCPQ), Université de Montréal (UdeM), Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC, Discovery grant RGPIN-2014-04595 to SJC) and by Cystic Fibrosis Canada [CF Canada grant ID number 2610 to RCL]. ATV holds an Alexander Graham Bell Canada Graduate Scholarships from the NSERC. SJC is a research scholar from the Fonds de Recherche du Québec en Santé. |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] adaptation medicine.disease_cause Dental Equipment MESH: Quorum Sensing Applied Microbiology and Biotechnology Genome Insertion sequence MESH: Water Supply MESH: Phylogeny MESH: Bacterial Proteins Phylogeny MESH: Gene Expression Regulation Bacterial Ecology Phylogenetic tree O Antigens Quorum Sensing MESH: Transcription Factors MESH: DNA Transposable Elements Pseudomonas aeruginosa MESH: Pseudomonas aeruginosa MESH: Dental Equipment insertion sequence MESH: Trans-Activators 030106 microbiology MESH: Biofilms Biology Microbiology 03 medical and health sciences Bacterial Proteins Water Supply Phylogenetics Drug Resistance Bacterial MESH: Drug Resistance Bacterial genomics medicine Gene MESH: O Antigens dental unit waterlines Biofilm Gene Expression Regulation Bacterial LasR Quorum sensing Biofilms DNA Transposable Elements Trans-Activators Transcription Factors |
| Zdroj: | FEMS Microbiology Ecology FEMS Microbiology Ecology, Wiley-Blackwell, 2017, 93 (9), ⟨10.1093/femsec/fix106⟩ |
| ISSN: | 1574-6941 0168-6496 |
| DOI: | 10.1093/femsec/fix106 |
| Popis: | International audience; The bacterium Pseudomonas aeruginosa is well known to have a remarkable adaptive capacity allowing it to colonise many environments. A recent study on environmental isolates from dental unit waterlines (DUWLs) hinted at a genetic clustering into two groups. Isolates from one of these groups, named cluster III, were shown to have unusual phenotypes for environmental isolates, such as an increased biofilm production. To have a better ecological view, more specifically on isolates from cluster III, the complete genomes of 39 isolates including 16 from DUWLs were sequenced. In addition to an investigation of antibiotic resistance and secretion system gene content, a molecular phylogeny allowed confirmation of the split of the 16 environmental isolates in two groups and also sheds light on a correlation between the phylogenetic positions and the serotypes of the isolates. Isolates from cluster III harboured insertion sequences ISPa11 inserted into the O-specific antigen biosynthesis clusters and the gene lasR, encoding for a master regulator of the quorum sensing. Investigation of key regulators revealed another truncated gene, gacS. Alteration in lasR and gacS genes was consistent with phenotypic assays confirming their inactivation. These results bring new perspectives regarding the ecological adaptive potential of P. aeruginosa. |
| Databáze: | OpenAIRE |
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