pKBuS13, a KPC-2-Encoding Plasmid from Klebsiella pneumoniae Sequence Type 833, Carrying Tn 4401b Inserted into an Xer Site-Specific Recombination Locus
| Autor: | Marina Busetti, Enrico Angelo Tonin, Luigi Garbari, Anna Knezevich, Raffaela Bressan, Fabrizia Gionechetti, Vincenzo Petix, Cristina Lagatolla, Lucilla Dolzani |
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| Přispěvatelé: | Garbari, Luigi, Busetti, Marina, Dolzani, Lucilla, Petix, Vincenzo, Knezevich, Anna, Bressan, Raffaela, Gionechetti, Fabrizia, Tonin, ENRICO ANGELO, Lagatolla, Cristina |
| Rok vydání: | 2015 |
| Předmět: |
Transposable element
Klebsiella pneumoniae Locus (genetics) Microbial Sensitivity Tests medicine.disease_cause Xer locus Epidemiology and Surveillance Microbiology sequence type 833 Plasmid Escherichia coli medicine Pharmacology (medical) Site-specific recombination transposon Tn4401 Gene Pharmacology Genetics ColE1 biology blaKPC-2 multimers biology.organism_classification Anti-Bacterial Agents Infectious Diseases DNA Transposable Elements blaKPC-2 transposon Tn4401 sequence type 833 Xer locus multimers Plasmids |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 59:5226-5231 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.04543-14 |
| Popis: | Here, we report the first detection of a Klebsiella pneumoniae carbapenemase 2 (KPC-2)-producing Klebsiella pneumoniae strain belonging to sequence type 833 (ST833), collected in an Italian hospital from a patient coming from South America. Its bla KPC determinant was carried by a ColE1 plasmid, pKBuS13, that showed the Tn 4401b :: bla KPC-2 transposon inserted into the regulatory region of an Xer site-specific recombination locus. This interfered with the correct resolution of plasmid multimers into monomers, lowering plasmid stability and leading to overestimation of the number of plasmids harbored by a single host cell. Sequencing of the fragments adjacent to Tn 4401b detected a region that did not have significant matches in databases other than the genome of a carbapenem-resistant Escherichia coli strain collected during the same year at a hospital in Boston. This is interesting in an epidemiologic context, as it suggests that despite the absence of tra genes and the instability under nonselective conditions, the circulation of pKBuS13 or of analogous plasmids might be wider than reported. |
| Databáze: | OpenAIRE |
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