Voltammetric determination of the phytoestrogen genistein in soy flours and soy based supplements using cationic surfactant cetyltrimetylammonium bromide
| Autor: | Valdir Souza Ferreira, Daniela Kárin Fogliatto, Antonio Marcos Jacques Barbosa |
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| Rok vydání: | 2010 |
| Předmět: |
Supporting electrolyte
Flour Genistein Phytoestrogens High-performance liquid chromatography Surface-Active Agents chemistry.chemical_compound Colloid and Surface Chemistry Pulmonary surfactant Bromide Cations Physical and Theoretical Chemistry Voltammetry Chromatography High Pressure Liquid Detection limit Chromatography Molecular Structure Cetrimonium Chemistry Extraction (chemistry) Reproducibility of Results Electrochemical Techniques Surfaces and Interfaces General Medicine Hydrogen-Ion Concentration Cetrimonium Compounds Soybeans Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 78:243-249 |
| ISSN: | 0927-7765 |
| Popis: | This paper proposes a methodology based on electrochemical reduction for a fast, specific and sensitive determination of genistein by square-wave voltammetry (SWV). The highest peak currents and optimal definition of reduction peak of genistein was obtained by using a supporting electrolyte consisted of 0.04 mol L(-1) phosphate buffer-methanol (8:2, v/v) solution at pH 7.5 and cationic surfactant 2.21 x 10(-4) mol L(-1) cetyltrimethylammonium bromide (CTAB). The peak current was proportional to the concentration of genistein in the range 1.14 x 10(-7) to 1.09 x 10(-6) mol L(-1), with a detection limit and quantification of 3.43 x 10(-8) mol L(-1) and 1.14 x 10(-7) mol L(-1), respectively. The extraction procedure was evaluated for a better determination of genistein contained in soy flour and soy based supplements. The efficiency of the proposed methodology was demonstrated by comparing the results obtained by SWV with those obtained by the high-performance liquid chromatography (HPLC) method. |
| Databáze: | OpenAIRE |
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