Adsorbed hollow fiber immobilized tyrosinase for the screening of enzyme inhibitors from Pueraria lobata extract
| Autor: | Cong-Peng Zhao, Shi-Jun Yin, Hua Chen, Feng-Qing Yang, Yuan Wang, Guo-Ying Chen, Jing Zhao |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Pueraria
Immobilized enzyme Tyrosinase Clinical Biochemistry Pharmaceutical Science 01 natural sciences Analytical Chemistry chemistry.chemical_compound Adsorption Puerarin Drug Discovery Enzyme Inhibitors IC50 Spectroscopy chemistry.chemical_classification Chromatography biology Monophenol Monooxygenase Plant Extracts 010405 organic chemistry Chemistry 010401 analytical chemistry Reproducibility of Results biology.organism_classification Isoflavones 0104 chemical sciences Molecular Docking Simulation Enzyme Kojic acid |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 193:113743 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2020.113743 |
| Popis: | In this study, a method based on adsorbed hollow fiber immobilized tyrosinase (TYR) was developed to screening potential TYR inhibitors from Pueraria lobate extract. Kojic acid and ranitidine were used as positive and negative control to verify the reliability of the proposed method, respectively. Several significant parameters of the screening process, including the amount of P. lobata extract, adsorption time and incubation time, were optimized. After investigating the repeatability of the developed method, seven potential active compounds in P. lobata extract were successfully detected and their chemical structures were tentatively identified by liquid chromatography - mass spectrometry analysis. Furthermore, the inhibitory activity of four identified compounds on TYR was tested in vitro, and three of them, namely, puerarin, puerarin-6″-O-xyloside and puerarin apioside were verified to have good TYR inhibitory activity with IC50 value of 478.5, 513.8, and 877.3 μM, respectively. In addition, the molecular docking results indicated that these compounds could bind to the amino acid residues in TYR catalytic pocket. These results proved that the proposed method is a feasible approach for screening of TYR inhibitors from plant extract. |
| Databáze: | OpenAIRE |
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