Ecotoxicology inside the gut: impact of heavy metals on the mouse microbiome

Autor: Bruno Pot, Jérôme Breton, Peter Vandamme, Evie De Brandt, Benoît Foligné, Sébastien Massart
Přispěvatelé: Department of Bio-engineering Sciences
Rok vydání: 2013
Předmět:
Feces/microbiology
Gut flora
Ecotoxicology
MICROFLORA
Feces
Mice
RNA
Ribosomal
16S
Ingestion
Pharmacology (medical)
Cecum
Gastrointestinal tract
Mice
Inbred BALB C
Microbiota/drug effects
RNA
Ribosomal
16S/genetics
Denaturing gradient gel electrophoresis (DGGE)
biology
Microbiota
Poisoning
Environmental exposure
Heavy metal exposure
ULCERATIVE-COLITIS
Lactobacillaceae
GASTROINTESTINAL-TRACT
Female
HEALTH
16S pyrosequencing
Cadmium
Research Article
Gut microbiota
Microbiology
Turicibacter
CADMIUM
GRADIENT GEL-ELECTROPHORESIS
REVEALS
Cadmium/toxicity
medicine
Animals
Microbiome
Lead/toxicity
Pharmacology
STABILITY
Lactobacillaceae/genetics
Denaturing Gradient Gel Electrophoresis
Lachnospiraceae
Cecum/drug effects
Biology and Life Sciences
medicine.disease
biology.organism_classification
Poisoning/etiology
Heavy Metal Poisoning
Lead
COMMUNITIES
Dysbiosis
ALTERS
Zdroj: BMC Pharmacology & Toxicology
BMC PHARMACOLOGY & TOXICOLOGY
Scopus-Elsevier
ISSN: 2050-6511
1471-2210
Popis: BACKGROUND: The gut microbiota is critical for intestinal homeostasis. Recent studies have revealed the links between different types of dysbiosis and diseases inside and outside the intestine. Environmental exposure to pollutants (such as heavy metals) can also impair various physiological functions for good health. Here, we studied the impact of up to 8 weeks of oral lead and cadmium ingestion on the composition of the murine intestinal microbiome. RESULTS: Pyrosequencing of 16S RNA sequences revealed minor but specific changes in bacterial commensal communities (at both family and genus levels) following oral exposure to the heavy metals, with notably low numbers of Lachnospiraceae and high numbers levels of Lactobacillaceae and Erysipelotrichaceacae (mainly due to changes in Turicibacter spp), relative to control animals. CONCLUSIONS: Non-absorbed heavy metals have a direct impact on the gut microbiota. In turn, this may impact the alimentary tract and overall gut homeostasis. Our results may enable more accurate assessment of the risk of intestinal disease associated with heavy metal ingestion.
Databáze: OpenAIRE
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