Effects of a patient-derived de novo coding alteration of CACNA1I in mice connect a schizophrenia risk gene with sleep spindle deficits
| Autor: | Megan Fitzgerald, lingling Yang, Karena Yan, Kazuo Imaizumi, David S. Uygun, Soon-Wook Choi, David Baez-Nieto, Jen Q. Pan, Zhanyan Fu, Xiaohong Mao, Mario A. Arias-Garcia, Shaun Purcell, Thomas B. Nicholson, Andrew S. Allen, Guoping Feng, Jen M. Hope, James M. McNally, Qiangge Zhang, Ritchie E. Brown, Ayan Ghoshal, Robert E. Strecker, Violeta G. Lopez-Huerta |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology Sleep REM Sleep spindle Biology medicine.disease_cause Non-rapid eye movement sleep Article lcsh:RC321-571 Calcium Channels T-Type Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Genetic model medicine Animals Humans Missense mutation lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Biological Psychiatry Thalamic reticular nucleus Mutation Electroencephalography medicine.disease 3. Good health Psychiatry and Mental health 030104 developmental biology medicine.anatomical_structure Schizophrenia Sleep Haploinsufficiency Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Translational Psychiatry Translational Psychiatry, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2158-3188 |
| DOI: | 10.1038/s41398-020-0685-1 |
| Popis: | CACNA1I, a schizophrenia risk gene, encodes a subtype of voltage-gated T-type calcium channel CaV3.3. We previously reported that a patient-derived missense de novo mutation (R1346H) of CACNA1I impaired CaV3.3 channel function. Here, we generated CaV3.3-RH knock-in animals, along with mice lacking CaV3.3, to investigate the biological impact of R1346H (RH) variation. We found that RH mutation altered cellular excitability in the thalamic reticular nucleus (TRN), where CaV3.3 is abundantly expressed. Moreover, RH mutation produced marked deficits in sleep spindle occurrence and morphology throughout non-rapid eye movement (NREM) sleep, while CaV3.3 haploinsufficiency gave rise to largely normal spindles. Therefore, mice harboring the RH mutation provide a patient derived genetic model not only to dissect the spindle biology but also to evaluate the effects of pharmacological reagents in normalizing sleep spindle deficits. Importantly, our analyses highlighted the significance of characterizing individual spindles and strengthen the inferences we can make across species over sleep spindles. In conclusion, this study established a translational link between a genetic allele and spindle deficits during NREM observed in schizophrenia patients, representing a key step toward testing the hypothesis that normalizing spindles may be beneficial for schizophrenia patients. |
| Databáze: | OpenAIRE |
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