Identification of novel filament-forming proteins in Saccharomyces cerevisiae and Drosophila melanogaster
| Autor: | Chalongrat Noree, James E. Wilhelm, Brian K. Sato, Risa M. Broyer |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cytidine triphosphate
Saccharomyces cerevisiae Proteins Prions Protein Conformation 1.1 Normal biological development and functioning Recombinant Fusion Proteins Saccharomyces cerevisiae Eukaryotic Initiation Factor-2 Guanosine macromolecular substances Medical and Health Sciences chemistry.chemical_compound Underpinning research Report Glutamate synthase Animals Drosophila Proteins Carbon-Nitrogen Ligases Translation factor Enzyme Inhibitors Research Articles Neurons Protein Synthesis Inhibitors biology ATP synthase fungi food and beverages Cell Biology Biological Sciences biology.organism_classification Staurosporine Nucleotidyltransferases Eukaryotic Initiation Factor-2B Drosophila melanogaster chemistry Biochemistry Cytoplasm biology.protein Cytoophidium Generic health relevance Developmental Biology |
| Zdroj: | The Journal of cell biology, vol 190, iss 4 The Journal of Cell Biology |
| Popis: | A screen for GFP-tagged yeast proteins that can assemble into visible structures reveals four new filamentous structures in the cytoplasm formed by metabolic enzymes and translation factors. The discovery of large supramolecular complexes such as the purinosome suggests that subcellular organization is central to enzyme regulation. A screen of the yeast GFP strain collection to identify proteins that assemble into visible structures identified four novel filament systems comprised of glutamate synthase, guanosine diphosphate–mannose pyrophosphorylase, cytidine triphosphate (CTP) synthase, or subunits of the eIF2/2B translation factor complex. Recruitment of CTP synthase to filaments and foci can be modulated by mutations and regulatory ligands that alter enzyme activity, arguing that the assembly of these structures is related to control of CTP synthase activity. CTP synthase filaments are evolutionarily conserved and are restricted to axons in neurons. This spatial regulation suggests that these filaments have additional functions separate from the regulation of enzyme activity. The identification of four novel filaments greatly expands the number of known intracellular filament networks and has broad implications for our understanding of how cells organize biochemical activities in the cytoplasm. |
| Databáze: | OpenAIRE |
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