Toxigenic diversity of two different RAPD groups of Stachybotrys chartarum isolates analyzed by potential for trichothecene production and for boar sperm cell motility inhibition
| Autor: | Mirja Salkinoja-Salonen, Ludwig Niessen, J. Peltola, Kristian Fog Nielsen, Bruce B. Jarvis, Birgitte Andersen, E.M. Møller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Male
skimmelsvampe Stereochemistry Stachybotrys chartarum mikrobiologi Immunology Trichothecene Genes Fungal Sus scrofa Trichodiene synthase Stachybotrys indeklima indoor climate svampesporer Applied Microbiology and Biotechnology Microbiology Indoor fungi mycotoksiner 03 medical and health sciences RAPD Genotype Genetics Building Materials Animals Carbon-Carbon Lyases Molecular Biology Phylogeny 030304 developmental biology 0303 health sciences Metabolite profiles biology 030306 microbiology fungi microbiology Indoor environment UPGMA General Medicine Fungi imperfecti Mycotoxins biology.organism_classification Spermatozoa Random Amplified Polymorphic DNA Technique biology.protein Sperm Motility moulds Trichothecenes |
| Zdroj: | Peltola, J, Niessen, L, Fog Nielsen, K, Jarvis, B B, Andersen, B, Salkinoja-Salonen, M & Möller, E M 2002, ' Toxigenic diversity of two different RAPD groups of Stachybotrys chartarum isolates analyzed by potential for trichothecene production and for boar sperm cell motility inhibition ', Canadian Journal of Microbiology, vol. 48, no. 11, pp. 1017-1029 . Peltola, J, Niessen, L, Nielsen, K F, Jarvis, BB, Andersen, B, Salkinoja-Salonen, M & Moller, EM 2002, ' Toxigenic diversity of two different RAPD groups of Stachybotrys chartarum isolates analyzed by potential for trichothecene production and for boar sperm cell motility inhibition ', Canadian Journal of Microbiology . https://doi.org/10.1139/w02-101 |
| DOI: | 10.1139/w02-101 |
| Popis: | Thirty-one isolates of Stachybotrys chartarum from indoor and outdoor environments were analyzed for the presence of the trichodiene synthase (Tri5) gene, trichothecenes, boar sperm cell motility inhibition, and randomly amplified polymorphic DNA banding patterns (RAPDs). Twenty-two S. chartarum isolates tested positive for the Tri5 gene and nine were negative when tested using novel Tri5 gene-specific PCR primer pair. The Tri5 gene positive isolates contained satratoxins (five isolates) or the simple trichothecene, trichodermol (11 isolates). The Tri5 gene negative isolates did not produce satratoxins or trichodermol. Nineteen S. chartarum isolates, distributed among the Tri5 gene negative and positive groups, inhibited boar spermatozoan motility at concentrations of [Formula: see text]60 μg of crude cell extract/mL. The inhibition of motility was independent of satratoxins or atranones. Unweighted pair group method of arithmetic averages (UPGMA) cluster analysis of RAPD fragments clustered the 31 S. chartarum isolates in two distinct groups designated as RAPD groups 1 and 2. The grouping of S. chartarum isolates obtained by UPGMA cluster analysis of RAPD fragments was identical to the grouping obtained by Tri5 gene-specific PCR. This indicates that the S. chartarum isolates belonging to different groups were genetically distinct in a much wider area than just the Tri5 gene.Key words: boar sperm cell motility inhibition, RAPD, Stachybotrys chartarum, toxicity, trichothecene, trichodiene synthase (Tri5) gene. |
| Databáze: | OpenAIRE |
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