Secure and Sustainable Sourcing of Plant Tissues for the Exhaustive Exploration of Their Chemodiversity
| Autor: | Matheus Silva da Fonseca Diniz, Paulo Wender Portal Gomes, Emmanuelle Lautié, Hervé Rogez, Johan Carlos Costa Santiago, Luziane da Cunha Borges, Paulo Roberto da Costa Sá, Milton N. da Silva, C. Silva, Sylvain Darnet, Rhodin C. Joseph, Thais Fiusa, Viviane Magno do Nascimento, Júlio César da Silva Cardoso, Marcela Natalia Rocha de Castro, Jean A. Boutin, Abraão de Jesus Barbosa Muribeca, Alberto C. Arruda, Mara S.P. Arruda |
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| Rok vydání: | 2020 |
| Předmět: |
natural products
Pharmaceutical Science 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 surface sterilization 03 medical and health sciences analytical characterization lcsh:Organic chemistry plant tissue cultures Drug Discovery Physical and Theoretical Chemistry Axenic mass spectrometry 030304 developmental biology Biological Products leaf explants 0303 health sciences biology secondary metabolites business.industry 010401 analytical chemistry Organic Chemistry food and beverages Plants biology.organism_classification 0104 chemical sciences Biotechnology Plant Leaves Chemistry (miscellaneous) Chemical diversity Plant species Molecular Medicine business |
| Zdroj: | Molecules Volume 25 Issue 24 Molecules, Vol 25, Iss 5992, p 5992 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25245992 |
| Popis: | The main challenge of plant chemical diversity exploration is how to develop tools to study exhaustively plant tissues. Their sustainable sourcing is a limitation as bioguided strategies and dereplication need quite large amounts of plant material. We examine if alternative solutions could overcome these difficulties by obtaining a secure, sustainable, and scalable source of tissues able to biosynthesize an array of metabolites. As this approach would be as independent of the botanical origin as possible, we chose eight plant species from different families. We applied a four steps culture establishment procedure, monitoring targeted compounds through mass spectrometry-based analytical methods. We also characterized the capacities of leaf explants in culture to produce diverse secondary metabolites. In vitro cultures were successfully established for six species with leaf explants still producing a diversity of compounds after the culture establishment procedure. Furthermore, explants from leaves of axenic plantlets were also analyzed. The detection of marker compounds was confirmed after six days in culture for all tested species. Our results show that the first stage of this approach aiming at easing exploration of plant chemodiversity was completed, and leaf tissues could offer an interesting alternative providing a constant source of natural compounds. |
| Databáze: | OpenAIRE |
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