Discovery and Genetic Characterization of Novel Paramyxoviruses Related to the Genus Henipavirus in Crocidura Species in the Republic of Korea
| Autor: | Kyungmin Park, Heung-Chul Kim, Jin Won Song, Geum-Young Lee, Seung Hye Cho, Kijin Kim, Seungho Lee, Terry A. Klein, Jusun Hwang, Jingyeong Lee, Seung Ho Lee, Won-Keun Kim, Shailesh Budhathoki, Jin Sun No, Jong Woo Kim, Seungchan Cho, Chang-Sub Uhm |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Genetics
genetic characterization and diversity viruses RNA potential zoonosis Biology biology.organism_classification Genome Microbiology DNA sequencing Virus QR1-502 Infectious Diseases Crocidura Genus Virology next-generation sequencing Crocidura paramyxovirus Gene novel virus discovery Henipavirus |
| Zdroj: | Viruses, Vol 13, Iss 2020, p 2020 (2021) Viruses Volume 13 Issue 10 |
| ISSN: | 1999-4915 |
| Popis: | Paramyxoviruses, negative-sense single-stranded RNA viruses, pose a critical threat to human public health. Currently, 78 species, 17 genera, and 4 subfamilies of paramyxoviruses are harbored by multiple natural reservoirs, including rodents, bats, birds, reptiles, and fish. Henipaviruses are critical zoonotic pathogens that cause severe acute respiratory distress and neurological diseases in humans. Using reverse transcription-polymerase chain reaction, 115 Crocidura species individuals were examined for the prevalence of paramyxovirus infections. Paramyxovirus RNA was observed in 26 (22.6%) shrews collected at five trapping sites, Republic of Korea. Herein, we report two genetically distinct novel paramyxoviruses (genus: Henipavirus): Gamak virus (GAKV) and Daeryong virus (DARV) isolated from C. lasiura and C. shantungensis, respectively. Two GAKVs and one DARV were nearly completely sequenced using next-generation sequencing. GAKV and DARV contain six genes (3′-N-P-M-F-G-L-5′) with genome sizes of 18,460 nucleotides and 19,471 nucleotides, respectively. The phylogenetic inference demonstrated that GAKV and DARV form independent genetic lineages of Henipavirus in Crocidura species. GAKV-infected human lung epithelial cells elicited the induction of type I/III interferons, interferon-stimulated genes, and proinflammatory cytokines. In conclusion, this study contributes further understandings of the molecular prevalence, genetic characteristics and diversity, and zoonotic potential of novel paramyxoviruses in shrews. |
| Databáze: | OpenAIRE |
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