Intracellular Movement of Green Fluorescent Protein–Tagged Phosphatidylinositol 3-Kinase in Response to Growth Factor Receptor Signaling
| Autor: | William J. Gullick, Rainer Pepperkok, Helen Gillham, Matthew Golding |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
green fluorescent protein
Cytoplasm phosphatidylinositol 3-kinase Cytochalasin D Receptor ErbB-3 Morpholines Recombinant Fusion Proteins Green Fluorescent Proteins Phosphatidylinositol 3-Kinases Biology Transfection Cell Line Cell membrane src Homology Domains chemistry.chemical_compound Mice Growth factor receptor Epidermal growth factor Proto-Oncogene Proteins medicine erbB-3 Animals Humans Phosphatidylinositol Phosphorylation Phosphoinositide-3 Kinase Inhibitors Epidermal Growth Factor Kinase Cell Membrane tyrosine kinase Biological Transport Cell Biology Tyrphostins Actin cytoskeleton Cell biology ErbB Receptors Luminescent Proteins medicine.anatomical_structure chemistry Microscopy Fluorescence Chromones focal complexes Quinazolines Original Article Signal transduction Protein Tyrosine Phosphatases Signal Transduction |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Phosphatidylinositol 3-kinase (PI 3-kinase) is a lipid kinase which has been implicated in mitogenesis, protein trafficking, inhibition of apoptosis, and integrin and actin functions. Here we show using a green fluorescent protein–tagged p85 subunit that phosphatidylinositol 3-kinase is distributed throughout the cytoplasm and is localized to focal adhesion complexes in resting NIH-3T3, A431, and MCF-7 cells. Ligand stimulation of an epidermal growth factor receptor/c-erbB-3 chimera expressed in these cells results in a redistribution of p85 to the cell membrane which is independent of the catalytic activity of the enzyme and the integrity of the actin cytoskeleton. The movement is, however, dependent on the phosphorylation status of the erbB-3 chimera. Using rhodamine-labeled epidermal growth factor we show that the phosphatidylinositol 3-kinase and the receptors colocalize in discrete patches on the cell surface. Low concentrations of ligand cause patching only at the periphery of the cells, whereas at high concentrations patches were seen over the whole cell surface. Using green fluorescent protein–tagged fragments of p85 we show that binding to the receptor requires the NH2-terminal part of the protein as well as its SH2 domains. |
| Databáze: | OpenAIRE |
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