5-Aminolevulinic Acid Triggered by Ultrasound Halts Tumor Proliferation in a Syngeneic Model of Breast Cancer
| Autor: | Loredana Serpe, E. Biasibetti, Giulia Gola, Gianni Durando, Roberto Canaparo, Iside Bruni, Andrea Francovich, Federica Foglietta, Maria Teresa Capucchio |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cell cycle checkpoint
Pharmaceutical Science medicine.disease_cause Article Breast cancer Pharmacy and materia medica breast cancer In vivo Drug Discovery medicine sonodynamic therapy business.industry ultrasound Sonodynamic therapy Cancer medicine.disease RS1-441 Apoptosis 5-aminolevulinic acid Cancer cell Cancer research Molecular Medicine Medicine business Oxidative stress |
| Zdroj: | Pharmaceuticals Volume 14 Issue 10 Pharmaceuticals, Vol 14, Iss 972, p 972 (2021) |
| Popis: | Sonodynamic therapy is a bimodal therapeutic approach in which a chemical compound and ultrasound (US) synergistically act to elicit oxidative damage, triggering cancer cell death. Despite encouraging results, mainly for anticancer treatment, sonodynamics is still far from having a clinical application. Therefore, to close the gap between the bench and bedside, more in vivo studies are needed. In this investigation, the combined effect of 5-aminolevulinic acid (Ala), a natural porphyrin precursor, plus exposure to US, was investigated in vivo on a syngeneic breast cancer model. Real-time RT-PCR, Western blotting, and immunohistochemistry assays were performed to evaluate the effect of sonodynamic treatment on the main cancer hallmarks. The sonodynamic-treated group had a significant reduction (p ≤ 0.0001) in tumor size compared to the untreated group, and the Ala- and US-only treated groups, where a strong decrease (p ≤ 0.0001) in Ki67 protein expression was the most relevant feature of sonodynamic-treated cancer tissues. Moreover, oxidative stress was confirmed as the pivotal driver of the anticancer effect through cell cycle arrest, apoptosis, and autophagy thus, sonodynamics should be explored further for cancer treatment. |
| Databáze: | OpenAIRE |
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