A Novel Peptide-Based Antimicrobial Wound Treatment is Effective Against Biofilms of Multi-Drug Resistant Wound Pathogens

Autor:	Mina Izadjoo, Neff Jennifer A, Hosan Kim, Bayramov Danir F, Esha A Patel, Li Zhenghao
Rok vydání:	2018
Předmět:	Antimicrobial peptides Peptide Microbial Sensitivity Tests 02 engineering and technology Drug resistance medicine.disease_cause Microbiology 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Anti-Infective Agents Materials Testing Humans Medicine chemistry.chemical_classification biology business.industry Pseudomonas aeruginosa Public Health Environmental and Occupational Health Biofilm General Medicine 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification Bandages Drug Resistance Multiple chemistry Staphylococcus aureus Biofilms Wound Infection Wounds and Injuries 0210 nano-technology business Bacteria Antimicrobial Cationic Peptides
Zdroj:	Military Medicine. 183:481-486
ISSN:	1930-613X 0026-4075
DOI:	10.1093/milmed/usx135
Popis:	Wound infections are a common complication of combat-related injuries that significantly increase morbidity and mortality. Multi-drug resistant (MDR) organisms and their associated biofilms play a significant role in the pathogenicity and chronicity of wound infections. A critical barrier to progress in the treatment of traumatic wounds is the need for broad spectrum antimicrobials that are effective against biofilms and compatible with topical delivery. In this study, we present the in vitro efficacy of two de novo designed cationic, antimicrobial peptides and related topical formulations against single species and polymicrobial biofilms of MDR bacteria. Minimum biofilm eradication concentrations for peptides ranged from 0.7 μM for Staphylococcus aureus to 13.2 μM for Pseudomonas aeruginosa. Varying pH did not adversely impact peptide activity, however, in the presence of albumin, minimum biofilm eradication concentrations generally increased. When formulated into gels or dressings, both peptides eradicated mono- and polymicrobial biofilms of MDR pathogens. The biocompatibility index (BI) was found to be greater than one for both ASP-1 and ASP-2, with a slightly greater (more favorable) BI for ASP-2. The BIs for both peptides were greater than BIs previously reported for commonly used topical antimicrobial agents. The antimicrobial peptides and related formulations presented provide a promising platform for treatment of wound biofilms to improve outcomes for those injured in combat.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ceb3cecf7e9feec9860b94f0d05a8757 https://doi.org/10.1093/milmed/usx135 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.