A positive feedback loop between progesterone and microsomal prostaglandin E synthase-1-mediated PGE2 promotes production of both in mouse granulosa cells
| Autor: | Kazuhiro Tamura, Takahiko Hara, Hiroaki Naraba, Kota Nakamura, Mikihiro Yoshie, Eiichi Tachikawa, Hiroshi Kogo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Agonist medicine.medical_specialty Physiology medicine.drug_class medicine.medical_treatment Primary Cell Culture Biology Biochemistry Dinoprostone Mice 03 medical and health sciences Genes Reporter Microsomes Internal medicine medicine Animals Luciferase Secretion Prostaglandin E2 Luciferases Promoter Regions Genetic Autocrine signalling Receptor Progesterone Prostaglandin-E Synthases Feedback Physiological Pharmacology Granulosa Cells Steroidogenic acute regulatory protein Norgestrel Cell Biology Autocrine Communication 030104 developmental biology Endocrinology Gene Expression Regulation Tetradecanoylphorbol Acetate Female lipids (amino acids peptides and proteins) Receptors Progesterone Signal Transduction medicine.drug Prostaglandin E |
| Zdroj: | Prostaglandins & Other Lipid Mediators. 123:56-62 |
| ISSN: | 1098-8823 |
| Popis: | Microsomal prostaglandin E synthase-1 (mPGES-1) is primarily expressed in granulosa cells (GCs) in the preovulatory follicle. Both prostaglandin E2 (PGE2) and progesterone (P4) are implicated in various reproductive functions. Here, we demonstrate that mPges-1 may be a direct downstream target gene of the P4 receptor and P4-stimulated PGE2 secretion can stimulate P4 production in a newly generated mouse GC line (GtsT). Treatment of GtsT cells with a P4 receptor agonist, norgestrel, markedly increased mPGES-1 expression detected by RT-PCR analysis. PGE2 secretion measured by an enzyme-linked immunosorbent assay was enhanced by P4 treatment. Luciferase assays revealed that the proximal promoter region of the mPges-1 gene was responsible for the effects of P4 treatment. Conversely, PGE2 treatment stimulated P4 secretion, which coordinated with mRNA expression of steroidogenic acute regulatory protein. Taken together, P4 may regulate mPGES-1 expression to increase PGE2 secretion and in turn P4 production. An autocrine loop between P4 and PGE2 might function to maintain the increased levels of both in GCs. |
| Databáze: | OpenAIRE |
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