A Novel PCR Assay for Quantification of HIV-1 RNA
| Autor: | Robert F. Siliciano, Joseph B. Margolick, Liang Shan, Hao Zhang, Evelyn E. Eisele, Gregory M. Laird, S. Alireza Rabi |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Reverse Transcriptase Polymerase Chain Reaction Immunology Pcr assay RNA-dependent RNA polymerase RNA HIV Infections Nuclease protection assay Biology Microbiology Virology Molecular biology Reverse transcriptase Hiv 1 rna Genome Replication and Regulation of Viral Gene Expression Reverse transcription polymerase chain reaction chemistry Insect Science HIV-1 Humans RNA Viral Nucleotide DNA Primers |
| Zdroj: | Journal of Virology. 87:6521-6525 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity. |
| Databáze: | OpenAIRE |
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