Molecular combing: A new tool in diagnosing leukemia
| Autor: | Antoine Ittel, Hélène Zattara, Nicolas Lévy, Charlene Chaix, Gérard Michel |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cancer Research Myeloid Chromosomes Human Pair 21 Chromosomal translocation Computational biology Biology Translocation Genetic 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine hemic and lymphatic diseases Genetics medicine Humans Child Gene Acute leukemia Chromosomes Human Pair 12 Proto-Oncogene Proteins c-ets Breakpoint General Medicine Precursor Cell Lymphoblastic Leukemia-Lymphoma Prognosis medicine.disease Repressor Proteins Leukemia ETV6 030104 developmental biology medicine.anatomical_structure Oncology RUNX1 chemistry Core Binding Factor Alpha 2 Subunit Cytogenetic Analysis Female Gene Fusion DNA Probes 030217 neurology & neurosurgery |
| Zdroj: | Cancer Biomarkers. 17:405-409 |
| ISSN: | 1875-8592 1574-0153 |
| Popis: | BACKGROUND According to the World Health Organization (WHO), recurrent cytogenetic abnormalities define many specific groups of hematopoietic tumors of acute myeloid and lymphoblastic leukemia, and these abnormalities are often strongly associated with prognosis and sometimes require specific treatments. These rearrangements are commonly detected by conventional and molecular cytogenetic techniques. OBJECTIVE Using an alternative method, we sought to highlight the presence of chromosomal rearrangements. METHODS We applied molecular combing to detect and directly visualize gene fusions associated with balanced translocations found in acute leukemia. RESULTS In patients harboring t(12;21)(p13;q22), we demonstrated the presence of the fusion using specific probes covering the ETV6 and RUNX1 genes, with a positive result occurring due to the hybridization of the two probes to the same DNA fiber. Thanks to molecular combing, we also showed the presence of different breakpoints using these same probes. CONCLUSIONS Using several probes that are specific to the most common genes involved in acute leukemia, molecular combing could be an interesting additional tool in acute leukemia diagnosis. |
| Databáze: | OpenAIRE |
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