Inhibitory effect of curcumin on angiogenesis in a streptozotocin-induced diabetic rat model: An aortic ring assay
| Autor: | Ehsan Zayerzadeh, Sara Faghihi-Kashani, Kourosh Kabir, Mohammad Hossein Dehghan, Hossein Mirmiranpour, Mehrdad Larry, Salume Salehi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Curcumin Diabetic rat Angiogenesis C-TC controls treated with curcumin cultured in the absence of VEGF lcsh:Medicine Aortic ring assay Matrix metalloproteinase DMSO dimethyl sulfoxide AP-1 activator protein 1 D-V diabetics in a culture containing VEGF C-V controls in the presence of VEGF 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Diabetes mellitus PBS phosphate buffer saline Aortic ring Internal medicine DM diabetes mellitus Medicine Inhibitory effect CPCSEA Committee for the Purpose of Control and Supervision of Experiments on Animals NF-kB nuclear factor kappa business.industry lcsh:R UPA urokinase plasminogen activator C controls in the absence of VEGF Streptozotocin medicine.disease VEGF VEGF vascular endothelial growth factor 030104 developmental biology Endocrinology Complementary and alternative medicine chemistry D-V-TC diabetics treated with curcumin in a culture containing VEGF 030220 oncology & carcinogenesis MMP matrix metalloproteinases Original Article business medicine.drug |
| Zdroj: | Journal of Traditional and Complementary Medicine Journal of Traditional and Complementary Medicine, Vol 6, Iss 4, Pp 437-441 (2016) |
| ISSN: | 2225-4110 |
| Popis: | Background Curcumin (diferuloylmethane) has been associated with the inhibition of angiogenesis, as well as the prevention of cancers and inflammatory processes. The aim of this study was to assess the efficacy of curcumin in suppressing angiogenesis in the cultured endothelial cells of rat aortic rings. Methods Eight-week-old male Wistar rats were randomized into five groups each with a different treatment and cell culturing paradigm: controls cultured in the absence of VEGF (vascular endothelial growth factor) (C), controls cultured in the presence of VEGF (C-V), controls treated with curcumin and then cultured in media lacking VEGF (C-TC), diabetics cultured in media supplemented with VEGF (D-V) and diabetics treated with curcumin and then cultured in media supplemented with VEGF (D-V-TC). Each group consisted of 8 animals. Diabetes was induced in by streptozotocin (STZ; 60 mg/kg body weight, IV). After 8 weeks, animals were sacrificed and their aortas were excised. Ring-shaped explants were embedded in a 96-well culture plate. Angiogenesis response was measured by counting the number of primary microtubules in each well. Results Optic microscopy revealed that the D-V group had the highest number of microvessels, while angiogenesis was not observed in the C or C-TC groups. The number of primary microtubules was significantly lower in the D-V-TC group compared to the D-V group (P Graphical abstract |
| Databáze: | OpenAIRE |
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