Generation Of Transgenic Dairy Cattle Using ‘in vitro’ Embryo Production
| Autor: | Paul Krimpenfort, Adriana Rademakers, Will Eyestone, Adriaan van der Schans, Sandra van den Broek, Patricia Kooiman, Erika Kootwijk, Gerard Platenburg, Frank Pieper, Rein Strijker, Herman de Boer |
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| Rok vydání: | 1991 |
| Předmět: |
Transgene
Restriction Mapping Biomedical Engineering Bioengineering Biology Transfection Applied Microbiology and Biotechnology Animals Genetically Modified Andrology Embryonic and Fetal Development Pregnancy Animals Humans Microinjection Cells Cultured Dairy cattle Southern blot Genetics Caseins Embryo DNA Embryo Transfer Embryo transfer Genetically modified organism Blot Blotting Southern Lactoferrin Oocytes Molecular Medicine Cattle Female Biotechnology |
| Zdroj: | Nature Biotechnology. 9:844-847 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/nbt0991-844 |
| Popis: | We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome. |
| Databáze: | OpenAIRE |
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