Multiplex PCR for simultaneous detection of the most frequent T cell receptor-delta gene rearrangements in childhood ALL
| Autor: | C Hanel, Birgit Beyermann, C Linderkamp, Karlheinz Seeger, Günter Henze, Tillmann Taube, S Duda |
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| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Gene Rearrangement delta-Chain T-Cell Antigen Receptor Hematology Gene rearrangement Biology Precursor Cell Lymphoblastic Leukemia-Lymphoma Molecular biology Polymerase Chain Reaction law.invention Blotting Southern Oncology law Child Preschool Multiplex polymerase chain reaction Agarose gel electrophoresis Humans Multiplex Variants of PCR Child Nested polymerase chain reaction Polymerase chain reaction Southern blot |
| Zdroj: | Leukemia. 11(11) |
| ISSN: | 0887-6924 |
| Popis: | A rapid and simple multiplex polymerase chain reaction (PCR) is described that is capable of identifying the six most frequent rearrangements of the T cell receptor (TCR)-delta gene segments in childhood acute lymphoblastic leukemia (ALL). The PCR products amplified in a single reaction are of different size for each TCR-delta gene rearrangement. Therefore, they are readily and unambiguously distinguished after agarose gel electrophoresis and assigned to a specific V-D-J gene rearrangement. There is no need for labor-intensive and time-consuming Southern blot hybridization or nested PCR. To evaluate the multiplex assay we chose 45 DNA samples of childhood ALL analyzed beforehand for TCR-delta gene rearrangements by Southern blot and single PCR of which 30 showed TCR-delta gene rearrangements. The multiplex PCR results corresponded to the Southern blot and single PCR analyses. The described multiplex PCR enables the detection of clonal markers in about 50% of patients in order to monitor minimal residual disease (MRD) in prospective studies with a high turnover of samples. |
| Databáze: | OpenAIRE |
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