Natural variation in ARF18 gene simultaneously affects seed weight and silique length in polyploid rapeseed
| Autor: | Xingchao Sun, Liang Zhang, Rong-Jun Li, Wei Hua, Hanzhong Wang, Linbin Deng, Zhiyong Hu, Xinfa Wang, Hongli Yang, Jing Liu |
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| Rok vydání: | 2015 |
| Předmět: |
Genetic Markers
Rapeseed Genetic Linkage Molecular Sequence Data Quantitative Trait Loci Quantitative trait locus Biology Polymerase Chain Reaction Polyploidy Polyploid Sequence Homology Nucleic Acid Amino Acid Sequence Transgenes Gene DNA Primers Plant Proteins Genetics Multidisciplinary Base Sequence Sequence Homology Amino Acid Brassica napus Brassica rapa fungi Maternal effect food and beverages Plants Genetically Modified Introns Alternative Splicing PNAS Plus Polygene Genetic marker Mutation Seeds Silique Transcriptome Gene Deletion Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 112 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1502160112 |
| Popis: | Seed weight (SW), which is one of the three major factors influencing grain yield, has been widely accepted as a complex trait that is controlled by polygenes, particularly in polyploid crops. Brassica napus L., which is the second leading crop source for vegetable oil around the world, is a tetraploid (4×) species. In the present study, we identified a major quantitative trait locus (QTL) on chromosome A9 of rapeseed in which the genes for SW and silique length (SL) were colocated. By fine mapping and association analysis, we uncovered a 165-bp deletion in the auxin-response factor 18 (ARF18) gene associated with increased SW and SL. ARF18 encodes an auxin-response factor and shows inhibitory activity on downstream auxin genes. This 55-aa deletion prevents ARF18 from forming homodimers, in turn resulting in the loss of binding activity. Furthermore, reciprocal crossing has shown that this QTL affects SW by maternal effects. Transcription analysis has shown that ARF18 regulates cell growth in the silique wall by acting via an auxin-response pathway. Together, our results suggest that ARF18 regulates silique wall development and determines SW via maternal regulation. In addition, our study reveals the first (to our knowledge) QTL in rapeseed and may provide insights into gene cloning involving polyploid crops. |
| Databáze: | OpenAIRE |
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