Purification and characterization of a chloride ion-dependent α-glucosidase from the midgut gland of Japanese scallop (Patinopecten yessoensis)

Autor: Wataru Saburi, Masayuki Okuyama, Taro Fukukawa, Atsuo Kimura, Haruhide Mori, Janjira Maneesan, Tetsushi Naraoka, Iizuka Takahisa, Hiroyuki Nakai, Takayoshi Tagami, Yasushi Masuda
Rok vydání: 2015
Předmět:
Zdroj: Bioscience, biotechnology, and biochemistry. 80(3)
ISSN: 1347-6947
Popis: Marine glycoside hydrolases hold enormous potential due to their habitat-related characteristics such as salt tolerance, barophilicity, and cold tolerance. We purified an α-glucosidase (PYG) from the midgut gland of the Japanese scallop (Patinopecten yessoensis) and found that this enzyme has unique characteristics. The use of acarbose affinity chromatography during the purification was particularly effective, increasing the specific activity 570-fold. PYG is an interesting chloride ion-dependent enzyme. Chloride ion causes distinctive changes in its enzymatic properties, increasing its hydrolysis rate, changing the pH profile of its enzyme activity, shifting the range of its pH stability to the alkaline region, and raising its optimal temperature from 37 to 55 °C. Furthermore, chloride ion altered PYG’s substrate specificity. PYG exhibited the highest Vmax/Km value toward maltooctaose in the absence of chloride ion and toward maltotriose in the presence of chloride ion.
Databáze: OpenAIRE