Vanillin selectively modulates the action of antibiotics against resistant bacteria
Autor: | Cicera Janaine Camilo, Thiago Sampaio de Freitas, Maria Karollyna do Nascimento Silva, Jaime Ribeiro-Filho, Henrique Douglas Melo Coutinho, Camila Fonseca Bezerra |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Imipenem Staphylococcus aureus medicine.drug_class Tetracycline Antibiotics Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology 03 medical and health sciences 0302 clinical medicine Drug Resistance Multiple Bacterial medicine Escherichia coli Norfloxacin Bacteria Pseudomonas aeruginosa Drug Synergism Antimicrobial Anti-Bacterial Agents Drug Combinations 030104 developmental biology Infectious Diseases 030220 oncology & carcinogenesis Benzaldehydes Gentamicin medicine.drug |
Zdroj: | Microbial pathogenesis. 113 |
ISSN: | 1096-1208 |
Popis: | The treatment of infections caused by microorganisms that are resistant to antibiotics represent one of the main challenges of medicine today, especially due to the inefficacy of long-term drug therapy. In the search for new alternatives to treat these infections, many researchers have been looking for new substances derived from natural products to replace, or be used in combination with conventional antibiotics. Vanillin is a phenolic compound whose antimicrobial activity has been used in the elimination of pathogens present in fruits and vegetables. However, its antibacterial and modulating properties remain to be characterized. Therefore, this work aimed to evaluate the antibacterial activity and analyze the modulator activity of vanillin in association with conventional antibiotics. The antimicrobial activity of vanillin was evaluated using the microdilution method to determine the Minimum Inhibitory Concentration (MIC) Standard strains of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and multi-resistant strains of Escherichia coli 06, Staphylococcus aureus 10, Pseudomonas aeruginosa 24 were used in this study. The antibiotic modulating effect was analyzed by combining vanillin with Norfloxacin, Imipenem, Gentamicin, Erythromycin and Tetracycline against the following multiresistant bacteria strains: Escherichia coli 06, Staphylococcus aureus 10 and Pseudomonas aeruginosa 24. Data were analyzed using the ANOVA test of two tracks followed by the post hoc Bonferroni test. Vanillin presented CIMs ≥1024μg/mL against all tested strains demonstrating that it did not present significant antibacterial activity. However, modulated the activity of gentamicin and imipenem against S. aureus and E. coli, causing a synergistic effect, but did not affect the activity of norfloxacin, tetracycline and erythromycin against these same microorganisms. A synergistic effect was also obtained from the association of vanillin with norfloxacin against P. aeruginosa. On the other hand, against this strain the association of vanillin with tetracycline and erythromycin caused antagonism, although the activity of gentamicin and imipenem was not affected. In conclusion, vanillin selectively modulated the activity of antibiotics against multiresistant bacteria and as such, might be useful in the development of new therapies against resistant microorganism. |
Databáze: | OpenAIRE |
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