The Chromatin-Associated Protein PWO1 Interacts with Plant Nuclear Lamin-like Components to Regulate Nuclear Size
Autor: | Pawel Mikulski, Yvonne Stahl, Kalyanikrishna, Daniel Schubert, Sara Farrona, Cezary Smaczniak, Gerco C. Angenent, Mareike L. Hohenstatt, Kerstin Kaufmann |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Heterochromatin Arabidopsis Polycomb-Group Proteins Plant Science Biology 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Life Science Constitutive heterochromatin Inner membrane Laboratorium voor Moleculaire Biologie Research Articles Cell Nucleus Regulation of gene expression Nuclear Lamina Arabidopsis Proteins fungi Nuclear Proteins Cell Biology biology.organism_classification Chromatin Lamins Cell biology Phenotype 030104 developmental biology Cell Nucleus Size Nuclear lamina Laboratory of Molecular Biology Carrier Proteins Lamin 010606 plant biology & botany |
Zdroj: | The Plant Cell, 31(5), 1141-1154 The Plant Cell 31 (2019) 5 |
ISSN: | 1040-4651 |
Popis: | Spatial organization of chromatin contributes to gene regulation of many cellular processes and includes a connection of chromatin with the nuclear lamina (NL). The NL is a protein mesh that resides underneath the inner nuclear membrane and consists of lamins and lamina-associated proteins. Chromatin regions associated with lamins in animals are characterized mostly by constitutive heterochromatin, but association with facultative heterochromatin mediated by Polycomb-group (PcG) proteins has been reported as well. In contrast with animals, plant NL components are largely not conserved and NL association with chromatin is poorly explored. Here, we present the connection between the lamin-like protein, CROWDED NUCLEI1 (CRWN1), and the chromatin- and PcG-associated component, PROLINE-TRYPTOPHANE-TRYPTOPHANE-PROLINE INTERACTOR OF POLYCOMBS1, in Arabidopsis (Arabidopsis thaliana). We show that PWO1 and CRWN1 proteins associate physically with each other, act in the same pathway to maintain nuclear morphology, and control expression of a similar set of target genes. Moreover, we demonstrate that transiently expressed PWO1 proteins form foci located partially at the subnuclear periphery. Ultimately, as CRWN1 and PWO1 are plant-specific, our results argue that plants might have developed an equivalent, rather than homologous, mechanism of linking chromatin repression and NL. |
Databáze: | OpenAIRE |
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