Restructuring of the plasma membrane upon damage by LC3-associated macropinocytosis
| Autor: | Catarina Dias, Adam Cohen Simonsen, Anne Sofie Busk Heitmann, Swantje Christin Häger, Stine Lauritzen Sønder, Jesper Nylandsted, Lisa B. Frankel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Multidisciplinary Chemistry Pinocytosis Cell Autophagy Autophagy-related protein 13 ULK1 Cell biology 03 medical and health sciences 0302 clinical medicine Membrane medicine.anatomical_structure Cytoplasm medicine 030217 neurology & neurosurgery Homeostasis 030304 developmental biology |
| Zdroj: | Sønder, S L, Häger, S C, Heitmann, A S B, Frankel, L B, Dias, C, Simonsen, A C & Nylandsted, J 2021, ' Restructuring of the plasma membrane upon damage by LC3-associated macropinocytosis ', Science Advances, vol. 7, no. 27, eabg1969 . https://doi.org/10.1126/sciadv.abg1969 |
| DOI: | 10.1126/sciadv.abg1969 |
| Popis: | The plasma membrane shapes and protects the eukaryotic cell from its surroundings and is crucial for cell life. Although initial repair mechanisms to reseal injured membranes are well established, less is known about how cells restructure damaged membranes in the aftermath to restore homeostasis. Here, we show that cells respond to plasma membrane injury by activating proteins associated with macropinocytosis specifically at the damaged membrane. Subsequent to membrane resealing, cells form large macropinosomes originating from the repair site, which eventually become positive for autophagy-related LC3B protein. This process occurs independent of ULK1, ATG13, and WIPI2 but dependent on ATG7, p62, and Rubicon. Internalized macropinosomes shrink in the cytoplasm, likely by osmotic draining, and eventually fuse with lysosomes. We propose that a form of macropinocytosis coupled to noncanonical autophagy, which we term LC3-associated macropinocytosis (LAM) functions to remove damaged material from the plasma membrane and restore membrane integrity upon injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|